Crosslinking Characteristics of an Genipin-Fixed Porcine Acellular Dermal Matrix

Abstract:

The determination of aldehyde in genipin(GP) was carried out through solution silver mirror reaction and curpous oxide reaction. Influence of various dosage, pH, temperature and reaction time on shrinkage temperature of GP-crosslinked porcine acellular dermal matrix (GP-pADM) were investigated. The cytotoxicity and cell morphology of GP-pADM were observed. The results reveal that the existence of aldehyde is proved by silver mirror reaction and curpous oxide reaction. With increasing dosage of GP, shrinkage temperature of pADM increase. And with increasing pH, temperature and time, shrinkage temperature exhibit an early ascending trend and then decline slightly. Cytotoxicity of GP-pADM is 0 grade with great morphology, cell L929 could adhesive grow on the surface and in the pore of this material. The implications of all this are that GP is an ideal biological crosslinking agent for biomaterials.

• Periodical: Applied Mechanics and Materials (Volumes 217-219)
• Pages: 969-974
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.217-219.969
• Online since: November 2012
• Authors: Nian Hua Dan, Shi Wei Xiao, Yang Hu, Wei Hua Dan
• Keywords: Acellular Dermal Matrix, Collagen, Crosslinking, Genipin

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