Three Dimensional Manipulation of Cells Using Holographic Optical Tweezers

Tao Tao; Jing Li; Yang Lin

doi:10.4028/www.scientific.net/AMM.241-244.513

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HomeApplied Mechanics and MaterialsApplied Mechanics and Materials Vols. 241-244Three Dimensional Manipulation of Cells Using...

Three Dimensional Manipulation of Cells Using Holographic Optical Tweezers

Abstract:

A holographic optical tweezers platform was built and a multi-plane adaptive-additive algorithm was used to generating holograms for the reconstruction of optical traps in three-dimensional (3D) spaces. Experiments of manipulating cells were conducted on such platform and complex 3D structures were built with yeast cells. The results demonstrate that holographic optical tweezers can manipulate groups of cells in three dimensions and effectively trap and separate cells in the direction of the optic axis without harming the cells. Based on those versatile functions, it is proved that holographic optical tweezers is a powerful tool for single cell analysis.

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Periodical:

Applied Mechanics and Materials (Volumes 241-244)

Pages:

513-516

DOI:

https://doi.org/10.4028/www.scientific.net/AMM.241-244.513

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Online since:

December 2012

Authors:

Tao Tao*, Jing Li, Yang Lin

Keywords:

Bio-Cell Manipulation, Holographic Optical Tweezers, Optical Trapping

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