Metabolic Characteristic Analysis of an Oil Field Wastewater Degrading Strain Chelatococcus G5

Abstract:

An oil field wastewater-degrading strain was isolated from the waste activated sludge of an oil field wastewater treatment plant. The degrading capabilities for oil field wastewater, culture conditions in a liquid enrichment medium, and metabolic characteristics for carbon sources in a Biolog-ECO microplate of this strain were investigated. The 16S rRNA gene sequences of the strain were very similar to each other (>99% similarity) and exhibited a similarity of 98% with other Chelatococcus sp. The COD removal efficiency of the oil field wastewater-degrading strain is 64.3%, based on a batch experiment after strain inoculation for 60 h. Chelatococcus G5 is a mesophilic and NaCl-tolerant strain that can be grown well at pH 7.0, 37 °C, and under shaking conditions. Its NaCl tolerance is up to 5.0%. Based on the metabolic characteristic analysis, up to 54.8% of the carbon sources in the Biolog-ECO microplate could be utilized and only 29.03% of these sources could be easily degraded by Chelatococcus G5.

• Periodical: Applied Mechanics and Materials (Volumes 260-261)
• Pages: 684-689
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.260-261.684
• Online since: December 2012
• Authors: Xin Yuan Liu, Can Wang, Min Ji
• Keywords: Biolog, Chelatococcus, Degradation Strain, Growth Characteristic, Oil Field Wastewater

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