The combined three-section crossbeam is an important component in the heavy gantry CNC milling-lathing machine tool and the dynamic characteristics are key precision factors for the machine tool. Three sections of the combined crossbeam are bolted by large industrial bolts and the influence of bolted joints should be evaluated in the dynamic analysis of the combined crossbeam. The dynamic characteristics of the combined three-section crossbeam were extracted by the modal experiment. The FEM of the monolithic crossbeam was modeled to analyze the dynamic characteristic parameters. The comparison of the analysis result and experimental result were shown. The experimental result matched well with the FEM of the monolithic crossbeam without bolted joints. As a result, the influence of the bolted joints could be ignorable in dynamic characteristic for the combined three-section crossbeam.