Fluorescent DNA Probes Based on Quantum Dots and Core/Shell Quantum Dots

Abstract:

Fluorescent DNA probes based on the fluorescence resonance energy transfer (FRET) were presented in this paper when AuNPs were as the energy acceptors and CdTe quantum dots and CdTe/SiO2 core/shell nanoparticles were as the energy acceptors, respectively. The DNA probes were prepared when energy donors and acceptors were conjugated with two single-stranded complementary oligonucleotides and hybridized with each other and the fluorescent intensity of probes could be decreased. The quenching efficiency of DNA probe was about 67 % when CdTe QDs were as the energy donors, while that of DNA probe was about 75 % when CdTe/SiO2 fluorescent core/shell nanoparticles were as the energy acceptors, which indicated that CdTe/SiO2 core/shell nanoparticles were suitable donors compared with CdTe QDs in DNA probe field.

• Periodical: Applied Mechanics and Materials (Volume 372)
• Pages: 115-118
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.372.115
• Online since: August 2013
• Authors: Meng Meng Song, Wen Juan Guo, Zhao Dai, Kai Li Qiu, Jun Fu Wei
• Keywords: Au Nanoparticles, Core/Shell, DNA Probe, FRET, Quantum Dot (QD)

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