Genetically Engineered Saccharomyces cerevisiae Strain that Can Ultilize Both Xylose and Glucose for Fermentation

Abstract:

The primary problem in producing fuel ethanol through microorganism fermentation with lignocellulose is the strain. We constructed a URA3-directed low copy integration-expression plasmid pZMYBX1 and rDNA-directed high copy integration-expression plasmid pZMYX2. Using the lithium acetate transformation method, we co-transformed the linearized plasmid pZMYBX1 (StuI) and pZMYX2 (HpaI) into the S. cerevisiae cells. Ultimately, we obtain three recombinants: HDY-ZMYWBG1, HDY-ZMYWBG2 and HDY-ZMYWBG3. The ethanol yield for HDY-ZMYWBG1 and HDY-ZMYWBG3 are 0.368 g/g and 0.365 g/g, respectively, which are higher than the 0.330 g/g yield for W5. This findings show that the xylose metabolic pathway could be introduced into the S. cerevisiae to produce an alternative strain for the production of biological ethanol from lignocellulose substrate.

• Periodical: Applied Mechanics and Materials (Volumes 448-453)
• Pages: 1637-1643
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.448-453.1637
• Online since: October 2013
• Authors: Jing Ping Ge, Lu Yan Zhang, Wen Xiang Ping*, Meng Yun Zhang, Yan Shen, Gang Song
• Keywords: Ethanol, Genetic Engineering, Glucose, Saccharomyces cerevisiae, Xylose

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