Immobilization of Papain on Regenerated Cellulose from Ionic Liquids

Ya Mei Yuan; Qiu Jin Li; Song Kun Yao; Wei Zhang; Jian Fei Zhang

doi:10.4028/www.scientific.net/AMM.448-453.1651

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HomeApplied Mechanics and MaterialsApplied Mechanics and Materials Vols. 448-453Immobilization of Papain on Regenerated Cellulose...

Immobilization of Papain on Regenerated Cellulose from Ionic Liquids

Abstract:

Films and beads of cellulose regenerated from room temperature ionic liquid 1-butyl-3-methyl imidazolium chloride ([BmiCl) were prepared. In this work, regenerated cellulose (RC) films and beads were modified with NaIO₄, and then papain was immobilized. The immobilized enzyme activities of RC carriers were determinated by BAEE (N-benzoyl-DL-arginine ethyl ester hydrochloride) method. According to the experiments, 5.0mg/mL of free papain was fixed to obtain the immobilized enzyme with high activity. Moreover, high content of cellulose regenerated from ILs (<saturated content) was benefit to achieve high activity of enzyme after immobilization.

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Periodical:

Applied Mechanics and Materials (Volumes 448-453)

Pages:

1651-1655

DOI:

https://doi.org/10.4028/www.scientific.net/AMM.448-453.1651

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Online since:

October 2013

Authors:

Ya Mei Yuan, Qiu Jin Li, Song Kun Yao, Wei Zhang, Jian Fei Zhang

Keywords:

Enzyme Activity, Ionic Liquid (IL), Papain Immobilization, Regenerated Cellulose

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