The Comparative Analysis of Two Homologous Genomic Islands Associated with the Noncoding Sequence in Enterobacter cloacae

Jiao Zhang; Lei Song

doi:10.4028/www.scientific.net/AMM.448-453.3

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The Comparative Analysis of Two Homologous Genomic Islands Associated with the Noncoding Sequence in Enterobacter cloacae
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HomeApplied Mechanics and MaterialsApplied Mechanics and Materials Vols. 448-453The Comparative Analysis of Two Homologous Genomic...

The Comparative Analysis of Two Homologous Genomic Islands Associated with the Noncoding Sequence in Enterobacter cloacae

Abstract:

The genomic islands (GIs) are usually the products of horizontal gene transfer (HGT) that is evolution pattern in prokaryote. Two homologous GIs (WSU1GI^Noand KU01GI^No) containing the homologous integrase of Bacteriophage P2 were determined in Enterobacter cloacae through flanking sequence alignment of the homologous integrase. The homologous GIs were integrated into the noncoding sequence. Their common flanking sequence is 5-AAGGCTCCCTCAGGAGC-3, and their integrases share 97% similarity. About two-thirds of the nucleotide sequences between WSU1GI^Noand KU01GI^No are highly similar. The different regions between WSU1GI^Noand KU01GI^No mainly include hypothetical gene, Phage-related tail gene, capsid gene, and baseplate assembly gene. In conclusion, the tandem arrangement of WSU1GI^Noand KU01GI^No will be artificially constructed because of their similar structural characteristics, and phage coat protein assembly will also be analyzed.

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Applied Mechanics and Materials (Volumes 448-453)

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3-6

DOI:

https://doi.org/10.4028/www.scientific.net/AMM.448-453.3

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Online since:

October 2013

Authors:

Jiao Zhang, Lei Song*

Keywords:

Comparative Analysis, Enterobacter cloacae, Genomic Island (GI), HP1 Integrase

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