Elution Separation of Polygonum Multiflorum Extract under HPLC Isocratic Elution

Tao Wang

doi:10.4028/www.scientific.net/AMM.455.33

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HomeApplied Mechanics and MaterialsApplied Mechanics and Materials Vol. 455Elution Separation of Polygonum Multiflorum...

Elution Separation of Polygonum Multiflorum Extract under HPLC Isocratic Elution

Abstract:

In this experiment, the effective compounds of Stilbene glucoside and anthraquinones from polygonum multiflorum extract were separated under HPLC isocratic elution. SinoChrom ODS-BP C18 is selected as chromatographic column. The optimal experimental conditions under 320nm and 254nm wavelength UV detector include the column temperature: 28°C, mobile phase: methanol/water, and flow rate: 1.0ml/mol. All as above, we can draw a conclusion that the technology of stilbene glucoside extraction is successful , the main ingredients of fleece-flower root extract can be effectively separated because the purity of the samples is extraordinary high. Simultaneously, this article provides significant reference values for the preparation and quality control of related new drugs.

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Periodical:

Applied Mechanics and Materials (Volume 455)

Pages:

33-38

DOI:

https://doi.org/10.4028/www.scientific.net/AMM.455.33

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Online since:

November 2013

Authors:

Tao Wang*

Keywords:

Anthraquinones, HPLC Isocratic Elution, Polygonum Multiflorum Extract, Stilbene Glucoside

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References

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DOI: 10.1016/s0031-9422(00)95282-8

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