Detection Device Construction and Analysis of Urinary Sediment

Abstract:

The purpose of this paper is to put up a device for urinary sediment detection with a homemade microscope and a CCD, and to test the feasibility of the device. In this study, we use a capillary to absorb the urinalysis control (UC) which is diluted 500 times, and drip it into a counting pool of a urinary sediment quantitative analysis board (USQAB). In this setup device, we detect 30 counting pools in this analysis board with microscopic examination in total. Further, we count the number of red blood cells (RBC) and white blood cells (WBC) of every counting pool, and calculate the average number of RBC and WBC of 30 counting pools. Though this detection device, we are able to get 30 groups of imaging results clearly while the number of RBC and WBC in each counting pool fluctuates around the mean value. The result indicates that the setup detection device in this experiment is simple and feasible.

• Periodical: Applied Mechanics and Materials (Volumes 462-463)
• Pages: 9-12
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.462-463.9
• Online since: November 2013
• Authors: Kai Xiang Li, Ji Hong Feng*, Hao He, Hua Juan Bai
• Keywords: Focusing, Microscope, Quantitative Analysis of Urinary Sediment, RBC and WBC, Urinalysis Control

References

[1] Fei Hu, Zhongyong Chang. Urinary dry chemical analyzer and analysis of white blood cell and red blood cell determined by urinary sediment [J]. International Journal of Laboratory Medicine, 2012, 33(11): 1391-1392. In Chinese.

[2] R.W. Chan, C.C. Szeto. Advances in the clinical laboratory assessment of urinary sediment [J]. Clinica Chimica Acta, 2004, 340: 67-78.

DOI: 10.1016/j.cccn.2003.11.006

[3] A. Costa, S. Iannelli, L.B. Desanctis, et al. Flow cytometry evaluation of urinary sediment in renal transplantation [J]. Transplant International, 1992, 5(Suppl 1): S8-S12.

DOI: 10.1111/tri.1992.5.s1.8

[4] Hongxia Zhang, Anxing Fei, Hong Jiang, Lingqiong Cao. Analysis of urinary white cell, red cell and tube type jointly determined by urinary dry chemical analysis, urinary sediment analyzer and microscopy [J]. Experimental and Laboratory Medicine, 2012, 30(3): 288-290. In Chinese.

[5] Xiuwen Yu, Juan Yao, Guoqing Wang. Review criteria for automatic complete blood count and WBC differential analysis by emergency laboratory SYSMEX XS-800I [J]. International Journal of Laboratory Medicine, 2011, 32(5): 557-559. In Chinese.

[6] Simona Verdesca, Claudia Brambilla, Giuseppe Garigali, et al. How a skillful and motivated urinary sediment examination can save the kidneys [J]. Nephrology Dialysis Transplantation, 2007, 22(6): 1778-1781.

DOI: 10.1093/ndt/gfm142

[7] Yingping Gao. Study on urinary sediment quantitative counting [J]. Chinese Journal of Trauma and Disability Medicine, 2012, 20(8): 82-83. In Chinese.

[8] Information on http: /www. beionmed. com/chn/art_fastread. asp.