Synthesis, Characterization and In Vitro Evaluation of Strontium-Containing Sol-Gel Derived Bioactive Glass/ Biphasic Calcium Phosphate Nanocomposite

Abstract:

In the present research, strontium containing nanobioactive glass (NBG-Sr) was synthesized by sol-gel method. The morphology was analyzed by transmission electron microscope (TEM). Different amounts (0.5 to 5 wt%) of NBG-Sr were then added to biphasic calcium phosphate (BCP). They were sintered at different temperatures, i.e., 1100, 1200 and 1300 °C and changes in physical and mechanical properties were investigated. A sharp decrease in pore volume was observed as the temperature increased. The maximum bending strength (~45 MPa) was achieved for BCP which was mixed with 3 wt% NBG-Sr and sintered at 1200 °C. This value was approximately the same when it was sintered at 1300 °C. The bending strength failed when both lower and higher amounts of 3 wt% NBG-Sr were utilized. Therefore, sintering of composites at 1200 °C was economically reasonable. The X-ray results showed that NBG-Sr additive did not change the phase composition of BCP when it was heat treated at 1200 °C. The attachment and proliferation of rat calvarium-derived osteoblasts on samples sintered at 1200 °C were also evaluated by scanning electron microscopy (SEM). Based on cell studies, all NBG-Sr-added BCPs supported attachment and proliferation of osteoblastic cells. Overall, biphasic calcium phosphate materials with improved mechanical and biological properties can be produced by using certain quantity of strontium-containing bioactive glass particles.

• Periodical: Applied Mechanics and Materials (Volume 467)
• Pages: 64-69
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.467.64
• Online since: December 2013
• Authors: Nader Nezafati*, Saeed Hesaraki, Mohammad-Reza Badr-Mohammadi
• Keywords: Biphasic Calcium Phosphate, Mechanical Property, Osteoblastic Cells, Sol-Gel Derived Nanobioactive Glass, Strontium

References

[1] J. Christoffersen, M.R. Christoffersen and N. Kolthoff: Bone 20 (1997), p.47.

[2] D.M. Chen, Y.F. Fu and G.Z. Gu: Chin J Biomed Eng. 20 (2003), p.278.

[3] R.Z. LeGeros, S. Lin, R. Rohanizadeh, D. Mijares and J.P. LeGeros: J mater sci Mater med. 14 (2003), p.201.

DOI: 10.1023/a:1022872421333

[4] S.V. Dorozhkin: Biomaterials 31 (2010), p.1465.

[5] M. Afzal, P. Kesarwani, K.M. Reddy, S. Kalmodia, B. Basu and K. Balani: Mater. Sci. Eng. C. 32 (2012), p.1164.

[6] M. Ashuri, F. Moztarzadeh, N. Nezafati, A. Ansari and M. Tahriri: Mater. Sci. Eng. C. 32 (2012), p.2330.

[7] N. Nezafati, F. Moztarzadeh, S. Hesaraki and M. Mozafari: Ceram. Int.: Volume 37 (2011), p.927.

[8] S. Hesaraki, M. Safari and M.A. Shokrgozar: J. Biomed. Mater. Res. B. Appl. Biomater. 91 (2009), p.459.

[9] G.X. Ni, K.Y. Chiu, W.W. Lu, Y. Wang, Y.G. Zhang and L.B. Hao; Biomaterials 27 (2006), p.4348.

[10] N. Kondo, A. Ogose and K. Tokunaga: Biomaterials 27 (2006), p.4419.

[11] J.R. Jones: Acta Biomater. 9 (2012), p.4457.

[12] S. Hesaraki, M.H. Barounian, S. Farhangdoust, M. Khorami, A. Zamanian and S. Borhan: Curr. Nanosci. 8 (2012), p.612.

DOI: 10.2174/157341312801784285

[13] S. Hesaraki, M. Alizadeh, H. Nazarian and D. Sharifi: J. Mater. Sci.: Mater. Med. 21(2010), p.695.

[14] M. Sila-Asna, A. Bunyaratvej, S. Maeda, H. Kitaguchi and N. Bunyaratavej: Kobe J Med Sci. 53 (2007), p.25.

[15] K. Qiu, X.J. Zhao, C.X. Wan, C.S. Zhao and Y.W. Chen: Biomaterials 27 (2006), p.1277.

[16] L. Morejón-Alonso, R.G. Carrodeguas, J.A.D. García-Menocal, J.A.A. Pérez and S.M. Manent: Mater. Res. 10 (2007), p.15.

[17] M.P. Ginebra, F.C.M. Driessens and J.A. Planell: Biomaterials 25 (2004), p.3453.

[18] A.M. Pietak, J.W. Reid, M.J. Stott and M. Sayer: Biomaterials 28 (2007), p.4023.

[19] K.L. Wong , C.T. Wong, W.C. Liu, H.B. Pan, M.K. Fong, W.M. Lam, W.L. Cheung, W.M. Tang, K.Y. Chiu, K.D.K. Luk and W.W. Lu: Biomaterials 30 (2009), p.3810.

DOI: 10.1016/j.biomaterials.2009.04.016

[20] G. X. Ni, Z. P. Yao, G. T. Huang, W. G. Liu and W. W. Lu: J. Mater. Sci.: Mater. Med. 22 (2011), p.961.