The Study of Oxymatrine on Apoptosis of Human Carcinoma Cell Line MGC-803 by Flow Cytometry

Yan Qin Zhu; Nan Nan Zheng; Lu Tang; Xi Feng Li

doi:10.4028/www.scientific.net/AMM.472.770

Paper Titles

One Step Growth of Semiconductor CdS Uniform Branched Nanowire on FTO
p.744

Study of MEMS Based Micropyrotechnic Igniter
p.750

A Review on Biodiesel Production: Breeding Technologies of Microalgae Containing Rich Lipid
p.759

The Anti-Influenza Activity of Cyclohexene Derivatives Studied by Topological Indices and Neural Network
p.764

The Study of Oxymatrine on Apoptosis of Human Carcinoma Cell Line MGC-803 by Flow Cytometry
p.770

Impact of Liquid Hot Water Pretreatment on the Structural Changes of Sugarcane Bagasse Biomass for Sugar Production
p.774

Mungongo Seeds Oil (Schinziophyton rautanenii) as a Potential Source of Bio-Diesel
p.780

Constituent Characteristic and Biomedical Potentials of Organic Essential Oils from Leaves of Four Eucalyptus Cultivars in China
p.785

The Changes of Ultrastructure and Proteome Induced by Repeated Eccentric Exercise on of Rat Skeletal Muscle
p.792

HomeApplied Mechanics and MaterialsApplied Mechanics and Materials Vol. 472The Study of Oxymatrine on Apoptosis of Human...

The Study of Oxymatrine on Apoptosis of Human Carcinoma Cell Line MGC-803 by Flow Cytometry

Abstract:

Objective:To study the effect of oxymatrine on apoptosis of human carcinoma cell line MGC-803. Methods:Flow cytometry were conducted to investigate apoptosis rate and expression of Bcl-2 and Bax protein. Results: At the dose of oxymatrine were 1mg/ml,2mg/ml,the apoptosis rates in MGC-803 were higher than those of controls (p<0.05), the expression of Bcl-2 was decreased and Bax was increased significantly (p<0.05).Conclusion:Oxymatrine induces apoptosis in human carcinoma cell line MGC-803, its mechanism includes up-regulated expression of Bax and down-regulated expression of Bcl-2.

You might also be interested in these eBooks

View Preview

Info:

Periodical:

Applied Mechanics and Materials (Volume 472)

Pages:

770-773

DOI:

https://doi.org/10.4028/www.scientific.net/AMM.472.770

Citation:

Cite this paper

Online since:

January 2014

Authors:

Yan Qin Zhu, Nan Nan Zheng, Lu Tang, Xi Feng Li

Keywords:

Apoptosis, Bax, Bcl-2, Carcinoma Cell Line, Flow Cytometry

Export:

RIS, BibTeX

Price:

Permissions CCC:

Request Permissions

Permissions PLS:

Request Permissions

Сopyright:

Citation:

References

[1] Y.Q. Zhu , F.X. Liu ,Y.F. Xu . Exper imental Study on Oxymatrine Inducing Apoptosis of Human Carcinom a Cells MGC-803 in Vivo and in Vitro . Chin Pharm J, 46(1) , 28~30(2011).

Google Scholar

[2] Z.Q. SiTu,J.Z. Wu. Cell culture . Xi`an: World Publishing Corporation, 78~88(2007).

Google Scholar

[3] MichaelG. Ormerod. Flow Cytometry. Oxford: Oxford University Press, 83~188(2010).

Google Scholar

[4] X. Wei . Growth inhibition and apoptosis induetion of two novel histone deacetylase inhibitors on human cervieal carcinoma cell lines in vitro . Central South University, 30~38(2012).

Google Scholar

[5] X.F. Guo ,Q. Gu ,N. Liu , et al. Relationship between apoptosis and change of P53 expression in mouse renal tissues after acute kidney injury. Chinese Journal of Pathophysiology 28( 11) : 1971~1975(2012).

Google Scholar

[6] Y.D. Liu ,Q. X Zheng , H.B. Wu , et al. The effects of rapamycin on expression ratio of Bax/Bcl-2 and the expression of activated caspase-3 in diferent types of tumor cells. TUMOR, 33(2) : 138~142(2013).

Google Scholar