The Changes of Ultrastructure and Proteome Induced by Repeated Eccentric Exercise on of Rat Skeletal Muscle

Abstract:

To research ultrastructure changes of rat skeletal muscle in the process of repetition eccentric exercise, Wistar rats were divided into normal control group, one eccentric exercise group, repeated eccentric exercise group. The ultrastructure and proteome changes made by downhill run and repetitive downhill run after a week in rats were observed in instant, 24h, 48h, 72h and 168h after one and repeated bout of exercise rats. The results showed that the most serious injuries of sarcomere in 48 hours after exercise, sarcomere disordered or disappeared, Z-line fractured and myofilament decomposed. From 24h to 48h, mitochondria was severely damaged, which structure gradually restored within 72 hours, the muscle fiber reconstructed, but mitochondrial number, structure and function had not yet been fully restored, the muscle cells in aerobic capacity have not yet fully been restored. At the same time, the injury severity of repeated exercise group was palliative compared to that of the first exercise, which may be related to recovery rate of mitochondrial damage. Therefore, repetitive motion can change the mitochondria's own repair speed, stimulate muscle to adapt the density of exercise, and reduce the extent of skeletal muscle micro-injury. Repeated eccentric exercise may promote key enzyme expression of energy metabolism and energy supply for damage cell, accelerate skeletal muscle contraction protein degradation and cell swallow, and scavenge free radicals and slow inflammatory reaction during 24-48h to speed up the skeletal muscle damage repair. Keywords: Eccentric Exercise, Repetitive Exercise, Skeletal Muscle, Ultrastructure, Peoteome

• Periodical: Applied Mechanics and Materials (Volume 472)
• Pages: 792-800
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.472.792
• Online since: January 2014
• Authors: Gui Jun Dong, Chen Xi Lv, Ke Feng Li*
• Keywords: Eccentric Exercise, Peoteome, Repetitive Exercise, Skeletal Muscle, Ultrastructure

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