Effects of Mechanical Stimulation on MMP-2 and TIMP-2 Expression of Scleral Fibroblasts after Posterior Sclera Reinforcement

Abstract:

To understand the effect of mechanical stimulation on posterior sclera reinforcement (PSR), the rabbit scleral fibroblasts after PSR were subjected to stretch in vitro and MMP-2 and TIMP-2 expression of scleral fibroblasts were evaluated. Three-week-old rabbits were monocularly performed by eyelid suturation randomly to prepare experimental myopia eye. After 60 days, the experimental myopia eyes were treated by PSR. After 6 months, the posterior pole scleral fibroblasts (normal sclera - group A, sclera after operation - group B and fusion region of sclera and reinforcing band group C) were isolated and cultured in vitro. The cells were subjected to cyclic stretch regimens (sine wave, 3% and 6% elongation amplitude, 0.1Hz, 48h duration) by FX-4000 Tension System. The MMP-2 and TIMP-2 expression of scleral fibroblasts were evaluated by ELISA method. The results show that after cyclic stretch to the scleral fibroblasts of the normal sclera and the sclera after operation, the MMP-2 expression was significantly reduced and the TIMP-2 expression was significantly increased, the MMP-2 and TIMP-2 expression of the scleral fibroblasts of the fusion region after operation was no changed. It was indicated that the mechanical stimulation could regulate the MMP-2 and TIMP-2 expression of scleral fibroblasts and play an important role in the process of treating high myopia with PSR surgery.

• Periodical: Applied Mechanics and Materials (Volumes 490-491)
• Pages: 867-871
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.490-491.867
• Online since: January 2014
• Authors: Guo Hui Wang, Wei Yi Chen
• Keywords: Mechanical Stimulation, MMP-2, Scleral Fibroblast, TIMP-2

References

[1] Cui, W., Bryant, M.T., Sweet, P.M., McDonnell, P.J., Changes in gene expression in response to mechanical strain in human scleral fibroblasts, Exp. Eye. Res. vol. 78, pp.275-284, (2004).

DOI: 10.1016/j.exer.2003.10.007

[2] Yamaoka, A., Matsuo, T., Shiraga, F., Ohtsuki. H. TIMP-1 production by human scleral fibroblasts decreases in response to cyclic mechanical stretching, Opohthalmic. Res. vol. 33, pp.98-101, (2001).

DOI: 10.1159/000055651

[3] Fujikura, H., Seko, Y., Tokoro, T., Mochizuki, M., Shimokawa, H., Involvement of mechanical stretch in the gelatinolytic activity of the fibrous sclera of chicks in vitro, Jpn. J. Ophthalmol. vol. 46, pp.24-30, (2002).

DOI: 10.1016/s0021-5155(01)00451-8

[4] Webb, K., Hitchcock, R.W., Smeal, R.M., Li, W., Gray, S.D., Tresco, P.A., Cyclic strain increases fibroblast proliferation, matrix accumulation, and elastic modulus of fibroblast-seeded polyurethane constructs, J. Biomech. vol. 39, p.1136–1144, (2006).

DOI: 10.1016/j.jbiomech.2004.08.026

[5] Shelton, L., Rada, J.S., Effects of cyclic mechanical stretch on extracellular matrix synthesis by human scleral fibroblasts, Exp. Eye. Res, vol. 84, pp.314-322, (2007).

DOI: 10.1016/j.exer.2006.10.004

[6] N. A. McBrien, A. Gentle, Role of the sclera in the development and pathological complications of myopia, Progress in Retinal and Eye Research, vol. 22, pp.307-338, (2003).

DOI: 10.1016/s1350-9462(02)00063-0

[7] Z. Zhu, X. Ji, J. Zhang, G. Ke, Posterior scleral reinforcement in the treatment of macular retinoschisis in highly myopic patients, Clin Experiment Ophthalmol, vol. 37, pp.660-663, (2009).

DOI: 10.1111/j.1442-9071.2009.02111.x

[8] R. Schippert, C. Brand, F. Schaeffel, M. P. Feldkaemper, Changes in scleral MMP-2, TIMP-2 and TGFβ-2 mRNA expression after imposed myopic and hyperopic defocus in chickens, Experimental Eye Research, vol. 82, p.710–719, (2006).

DOI: 10.1016/j.exer.2005.09.010

[9] Guohui Wang, Weiyi Chen, Effects of mechanical stimulation on viscoelasticity of rabbit scleral fibroblasts after posterior scleral reinforcement, Experimental Biology and Medicine, vol. 237, pp.1150-1154, (2012).

DOI: 10.1258/ebm.2012.012196

[10] P. Hu, J. H. Li, The study of the role of MMP–2, TIMP-2 and growth factors in lens-induced myopia, Chinese Journal of Optometry & Ophthalmology, vol. 10, pp.17-20, (2008).

[11] W.Y. Chen, X. J. Wang, C.Y. Wang, T. Li, X.N. Li, Q.Y. Zhang, An experimental study on collagen content and biomechanical properties of sclera after posterior sclera reinforcement, Clinical Biomechanics, vol. 23, p.17–20, (2008).

DOI: 10.1016/j.clinbiomech.2007.10.013

[12] Guohui Wang, Yongfang Xie, Weiyi Chen, The MMP-2 and TIMP-2 Expression of Sclera and Scleral Fibroblasts after Posterior Sclera Reinforcement Surgery, The 3rd International Conference on Biomedical Engineering and Informatics, vol. 5, pp.2134-2137, (2010).

DOI: 10.1109/bmei.2010.5639967

[13] Guohui Wang, Weiyi Chen, Xiaona Li. Advances in biomechanical study of sclera and scleral fibroblasts, Journal of Medical Biomechanics, vol. 25, pp.230-234 Jun (2010).

[14] T. H. Korver, R. J. Stadt, E. Kiljan, Effects of loading on the synthesis of proteoglycans in different layers of anatomically intact articular cartilage in vitro, J Rheumatol, vol. 19, pp: 905-912, (1992).

[15] Wang Guohui, Chen Weiyi, Xie Yongfang, Effects of mechanical stimulation on proliferation activity and CTGF expression of sclera fibroblasts, Journal of Medical Biomechanics, vol. 25, pp.230-234, (2010).