Expression of a Recombinant Endo-β-1,4-Xylanase in Pichia pastoris and its Application in Degradation of Corn Stalk

Abstract:

The optimization of xylanase expression by recombinant Pichia pastoris were carried out in this study. Several factors were evaluated and the conclusion were as follow: the optimal conditions were in shake flask cultivation with the rotate speed 180 r/min using BMGY medium with initial pH 7.0, initial OD₆₀₀ 1.0, 0.1% histidine, 0.05% tween80. 1.0% methanol was added into the culture every 24 h. The xylanase activity was up to 1527 U/mL at the optimal conditions after 15 days. The optimum pH and temperature were pH5.0 and 50°C. The recombinant xylanase was stable over a pH range of 2.0-8.0. The optimal conditions of degradation were as below: after 20 h, with the pH 5.0 and temperature 45°C, 2.0% of substrate concentration, 100 U/mL of enzyme dosage and 0.05% of tween80 concentration,the degradation of xylan was the best, which indicating great potential in the bioconversion of lignocellulosic waste to xylooligosaccharide.

• Periodical: Applied Mechanics and Materials (Volume 508)
• Pages: 279-285
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.508.279
• Online since: January 2014
• Authors: Qi Li, Li Ya Xi, Yi Yang Feng, Lin Guo Zhao*, Fei Li
• Keywords: Aspergillus niger, Degradation, Optimization, Pichia pastoris, Xylanase

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