Hybridization of ODNs with the Core-Shell Structure of Fe3O4 Nanoparticles and CS/ALG Composite Structure

Abstract:

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This study develops a nanocomposite structure with magnetism and biocompatibility. Composite structures with magnetism can be applied for biomarkers, specific tissue cell detection and targeted drug therapy. This study adopts a chemical disposition method to prepare Fe3O4 magnetic nanoparticles with the average size of 20-25nm. The complex biocompatible chitosan-alginate membrane covers Fe3O4 magnetic nanoparticles and the thickness of the complex membrane is controlled at 50-80nm. The efficiency of the oligonucleotide (ODN) combination is increased through the high biocompatibility of this composite film. Two groups of different sequences of ODNs and a bridge ODN undergo hybridization. The results show that the intensity at which the Fe3O4 is covered by chitosan-alginate composite film conjugated with ODNs is 2300nN. Furthermore, Fe3O4 covered by complex membrane of chitosan-alginate hybridized with 30 μM ODNs to yield the optimum hybridization intensity of 4528 nN, and the average hybridization intensity of ODNs with different concentration is 3971 nN.

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Periodical:

Edited by:

Zhou Mark

Pages:

349-353

DOI:

10.4028/www.scientific.net/AMM.52-54.349

Citation:

H. Chang et al., "Hybridization of ODNs with the Core-Shell Structure of Fe3O4 Nanoparticles and CS/ALG Composite Structure", Applied Mechanics and Materials, Vols. 52-54, pp. 349-353, 2011

Online since:

March 2011

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$35.00

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