Hybridization of ODNs with the Core-Shell Structure of Fe3O4 Nanoparticles and CS/ALG Composite Structure
This study develops a nanocomposite structure with magnetism and biocompatibility. Composite structures with magnetism can be applied for biomarkers, specific tissue cell detection and targeted drug therapy. This study adopts a chemical disposition method to prepare Fe3O4 magnetic nanoparticles with the average size of 20-25nm. The complex biocompatible chitosan-alginate membrane covers Fe3O4 magnetic nanoparticles and the thickness of the complex membrane is controlled at 50-80nm. The efficiency of the oligonucleotide (ODN) combination is increased through the high biocompatibility of this composite film. Two groups of different sequences of ODNs and a bridge ODN undergo hybridization. The results show that the intensity at which the Fe3O4 is covered by chitosan-alginate composite film conjugated with ODNs is 2300nN. Furthermore, Fe3O4 covered by complex membrane of chitosan-alginate hybridized with 30 μM ODNs to yield the optimum hybridization intensity of 4528 nN, and the average hybridization intensity of ODNs with different concentration is 3971 nN.
H. Chang et al., "Hybridization of ODNs with the Core-Shell Structure of Fe3O4 Nanoparticles and CS/ALG Composite Structure", Applied Mechanics and Materials, Vols. 52-54, pp. 349-353, 2011