Hybridization of ODNs with the Core-Shell Structure of Fe3O4 Nanoparticles and CS/ALG Composite Structure

Ho Chang; Yan Chyuan Wu; Chih Hao Chen; Ren Jei Chung; Kung Ching Cho; Wei Lin Lin

doi:10.4028/www.scientific.net/AMM.52-54.349

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HomeApplied Mechanics and MaterialsApplied Mechanics and Materials Vols. 52-54Hybridization of ODNs with the Core-Shell...

Hybridization of ODNs with the Core-Shell Structure of Fe₃O₄ Nanoparticles and CS/ALG Composite Structure

Abstract:

This study develops a nanocomposite structure with magnetism and biocompatibility. Composite structures with magnetism can be applied for biomarkers, specific tissue cell detection and targeted drug therapy. This study adopts a chemical disposition method to prepare Fe3O4 magnetic nanoparticles with the average size of 20-25nm. The complex biocompatible chitosan-alginate membrane covers Fe3O4 magnetic nanoparticles and the thickness of the complex membrane is controlled at 50-80nm. The efficiency of the oligonucleotide (ODN) combination is increased through the high biocompatibility of this composite film. Two groups of different sequences of ODNs and a bridge ODN undergo hybridization. The results show that the intensity at which the Fe3O4 is covered by chitosan-alginate composite film conjugated with ODNs is 2300nN. Furthermore, Fe3O4 covered by complex membrane of chitosan-alginate hybridized with 30 μM ODNs to yield the optimum hybridization intensity of 4528 nN, and the average hybridization intensity of ODNs with different concentration is 3971 nN.

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Applied Mechanics and Materials (Volumes 52-54)

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349-353

DOI:

https://doi.org/10.4028/www.scientific.net/AMM.52-54.349

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Online since:

March 2011

Authors:

Ho Chang, Yan Chyuan Wu, Chih Hao Chen, Ren Jei Chung, Kung Ching Cho, Wei Lin Lin

Keywords:

Chitosan-Alginate (CS/ALG), Fe₃O₄ Nanoparticle, Hybridisation, Nano-Indentator, Oligodeoxynucleotides (ODNs)

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