Distinguishing Single Nucleotide Polymorphisms of DNA Using Fluorescent Oligonucleotide Probe Containing 2-Aminopurine

Lin Bai; Hao Qin; Yu Guo Jiao

doi:10.4028/www.scientific.net/AMM.522-524.281

Paper Titles

Desorption Kinetics and Difference in Removal Enhancement of PAHs in Aged Soils by Tween 80
p.257

Effects of Elevated CO₂ and O₃ Concentrations on Isoprenoid Emissions from Pinus tabulaeformis
p.264

Identification and Bioinformatics Analysis of Cysteine Synthase from the Filamentous Fungus, Monascus purpureus
p.272

Impact of Inorganic Anions on Cadmium Fractions in the Soils
p.276

Distinguishing Single Nucleotide Polymorphisms of DNA Using Fluorescent Oligonucleotide Probe Containing 2-Aminopurine
p.281

Effect of NaCl Stress on the Photosynthetic Attributes of Atriplex centralasiatica
p.285

Effect of Phosphate and Sulfate on the Solubility of Na₂SO₄ in Supercritical Water
p.290

Plackett-Burman Design for Media Nutrients of Biocontrol Lysinibacillus xylanilyticus BPM1 against Aflatoxin
p.295

Proteomics Study on Heavy Metal Contaminated Bellamya aeruginosas in Poyang Lake
p.299

HomeApplied Mechanics and MaterialsApplied Mechanics and Materials Vols. 522-524Distinguishing Single Nucleotide Polymorphisms of...

Distinguishing Single Nucleotide Polymorphisms of DNA Using Fluorescent Oligonucleotide Probe Containing 2-Aminopurine

Abstract:

The fluorescence intensity of double-stranded DNA (ds-DNA) hybridized by fluorescent 2-aminopurine (2-AP) oligonucleotide probe and different mismatched bases was studied by fluorescence spectra in this paper. The experiment designed and synthesised four oligonucleotide sequences with the bases of adenine (A), cytosine (C), guanine (G), thymine (T), and determined the fluorescence intensity of the mismatched double-stranded DNA. The results implied that the fluorescence intensity of oligonucleotide probe was varied due to different mismatched bases. And the fluorescence intensity was 546.9 with the ratio of 3.13, which showed a significantly increase as the mismatched base was A. While the mismatched base was T, the fluorescence intensity quenched to 43.26, as the ratio was 0.25. For C and G, the fluorescence intensity of 2-AP was 99.14 and 89.03, respectively, which showed a different degree of reduction.

You might also be interested in these eBooks

Environmental Protection and Sustainable Development

View Preview

Info:

Periodical:

Applied Mechanics and Materials (Volumes 522-524)

Pages:

281-284

DOI:

https://doi.org/10.4028/www.scientific.net/AMM.522-524.281

Citation:

Cite this paper

Online since:

February 2014

Authors:

Lin Bai, Hao Qin, Yu Guo Jiao*

Keywords:

2-aminopurine, Fluorescence Intensity, Fluorescent Oligonucleotide Probe, Mismatched Base

Export:

RIS, BibTeX

Price:

Permissions CCC:

Request Permissions

Permissions PLS:

Request Permissions

Сopyright:

Citation:

* - Corresponding Author

References

[1] K. Nakatani: ChemBioChem Vol. 5 (2004), p.1623.

Google Scholar

[2] D.G. Wang, J.B. Fan, C.J. Siao, A. Berno, P. Young, R. Sapolsky, G. Ghandour, N. Perkins, E. Winchester, J. Spencer, L. Kruglyak, L. Stein, L. Hsie, T. Topaloglou, E. Hubbell, E. Robinson, M. Mittmann, M. S. Morris, N. Shen, D. Kilburn, J. Rioux, C. Nusbaum, S. Rozen, T. J. Hudson, R. Lipshutz, M. Chee and E.S. Lander: Science Vol. 280 (1998).

DOI: 10.1126/science.280.5366.1077

Google Scholar

[3] R. Yamada: Nat. Clin. Pract. Rheum. Vol. 4 (2008), p.210.

Google Scholar

[4] T. LaFramboise: Nucleic Acids Res. Vol. 37 (2009), p.4181.

Google Scholar

[5] S.J.O. Hardman and K.C. Thompson: Biochemistry-US Vol. 45 (2006), p.9145.

Google Scholar

[6] D.C. Ward, E. Reich and L. Stryer: J. Biol. Chem. Vol. 244 (1969), p.1228.

Google Scholar

[7] R. Buscaglia, D.M. Jameson and J.B. Chaires: Nucleic Acids Res. Vol. 40 (2012), p.4203.

Google Scholar

[8] T.J. Su, B.A. Connolly, C. Darlington, R. Mallin and D.T.F. Dryden: Nucleic Acids Res. Vol. 32 (2004), p.2223.

Google Scholar

[9] E.L. Rachofsky, R. Osman and J.B.A. Ross: Biochemistry-US Vol. 40 (2001), p.946.

Google Scholar

[10] C. Wan, T. Fiebig, O. Schiemann, J. Barton and A. Zewail: P. Natl. Acad. Sci. USA Vol. 97 (2000), p.14052.

Google Scholar

[11] J.X. Liang and S. Matsika: J. Am. Chem. Soc. Vol. 133 (2011), p.6799.

Google Scholar

[12] Y. Jiao, S. Stringfellow and H. Yu: J. Biomol. Struct. Dyn. Vol. 19 (2002), p.929.

Google Scholar