Plackett-Burman Design for Media Nutrients of Biocontrol Lysinibacillus xylanilyticus BPM1 against Aflatoxin

Abstract:

Aflatoxins (AFs) are a series of highly toxic and carcinogenic secondary metabolites. In order to eliminate AFs contamination, biological control is one of the more promising techniques. In this study, we describe the optimization of media nutrients for the selected biocontrol bacterium, Lysinibacillus xylanilyticus strain BPM1. The strain was isolated from the peanut hulls in Shandong Province, China and exhibited antagonistic activity against aflatoxins. Maltose and sucrose were identified as best carbon source, while soya peptone and yeast extract as nitrogen source led to the highest OD₆₀₀ observations. Medium composition was optimized using Plackett-Burman design, which was applied to find the key ingredients. The results revealed that the most significant two factors which were more effective in the fermentation of L. xylanilyticus BPM1 were soya peptone and yeast extract.

• Periodical: Applied Mechanics and Materials (Volumes 522-524)
• Pages: 295-298
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.522-524.295
• Online since: February 2014
• Authors: Kai Wang, Pei Sheng Yan*, Li Xin Cao
• Keywords: Aflatoxin, Biocontrol, Lysinibacillus xylanilyticus, Plackett-Burman

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