Low Light Affacting Alkaloids Accumulation and Related Biosynthetic Pathway Genes Expression in Leaves of Catharanthus roseus Seedlings

Abstract:

The alkaloids contents were investigated by High performance liquid chromatography (HPLC) in Catharanthus roseus aseptic seedlings leaves, the expressions of terpenoid indole alkaloids (TIAs) biosynthesis pathway genes were detected by semi-quantitative RT-PCR. The plastic films treatment induces a low light condition. HPLC analysis shows, the contents of vindoline (VIN) and catharanthine (CAT) are slight increase first and significant decrease (p<0.05) under plastic films, the vinblastine (VBL) content increase and reach the maximum (0.028 mg g^-1±0.0051) on the 15th day of treatment. TIA biosynthesis pathway genes expression change under low light. The correlation analysis indicates, VIN accumulation is significantly correlated with the gene expression of desacetoxyvindoline-4-hydroxylase (D4h) and deacetylvindoline-4-O-acetyl transferase (Dat), and CAT accumulation is significantly correlated with strictosidine synthase (Str) gene expression (p<0.05). The low light treatment with plastic films accelerates the accumulation of VBL in C. roseus seedlings leaves.

• Periodical: Applied Mechanics and Materials (Volumes 522-524)
• Pages: 311-315
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.522-524.311
• Online since: February 2014
• Authors: Ying Liu, De Wen Li*, Zhong Hua Zhang, Yuan Gang Zu
• Keywords: Alkaloids Content, C. roseus, Gene Expression, Low Light, Plastic Films

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