Cloning of MeCWINV3 Promoter from Cassava and Transient Expression Analysis in Tobacco

Abstract:

In order to study the inducement pattern and regulating mechanism of MeCWINV3 in Cassava. An 1160 bp promoter region upstream of the MeCWINV3 gene (GenBank Accession No. KC905170) was isolated from Cassava (Manihot esculenta) genomic DNA using PCR methods. Sequence analysis found that it contains typical TATA box and CAAT box, and several cis-acting elements that related plant stress responses, such as ABRE, ARFAT, GAREAT, MYB and MYC transcription factors. Furthermore, transient expression in transgenic tobacco was analyzed by inserting upstream of GUS gene in expressing vector. The results showed that GUS was mainly expressed in tobacco veins. This will be the basis for further investigating the function of the MeCWINV3 gene promoter.

• Periodical: Applied Mechanics and Materials (Volumes 522-524)
• Pages: 326-331
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.522-524.326
• Online since: February 2014
• Authors: Yan Ping Hu, Jiao Liu, Yang Zhou, Wen Rui Xia, Rui Mei Li, Rui Jun Duan, Shao Ping Fu, Xin Wen Hu, Jian Chun Guo
• Keywords: Cell Wall Acid Invertase, GUS Gene, Manihot esculenta, Promoter, Transient Expression

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