For Libraries For Publication Open Access Downloads About Us Contact Us
Paper Titles
Surfactant-Enhanced Washing of Aged PAH Contaminated Soils: Comparison between Nonionic Surfactant and Anionic Surfactant
p.316
Research on the Isolation, Identification and Degradation Characteristics of Two Hydrocarbon Degrading Strain
p.322
Cloning of MeCWINV3 Promoter from Cassava and Transient Expression Analysis in Tobacco
p.326
Expression of p38 MAPK in Acute Lung Injury Induced by LPS in Mice
p.332
Interaction of 4-Nitroaniline with Serum Albumin
p.337
Effect of Acid Stress on the Behavior of Soil Nematodes
p.341
Effects of pH and Temperature on the Degradation of Polycarbonate in Water
p.346
The Immunohistochemical Staining Results of Three Different Decalcified Liquid on Tooth Specimens
p.349
Perchlorate Removal in Biodegradable Polymers Bioreactor
p.353

Interaction of 4-Nitroaniline with Serum Albumin

Article Preview

Abstract:

Bovine serum albumin (BSA) and human serum albumin (HSA) interaction with 4-nitroaniline was investigated by fluorescence spectroscopy respectively. 4-Nitroaniline can strongly quench intrinsic fluorescence of BSA and HSA. 4-Nitroaniline exhibits a high affinity to bovine and human serum albumins. The binding constants K and the number binding site n were obtained by double-log regression equation. Negative enthalpy (ΔH) and positive entropy (ΔS) values indicated that both hydrogen bond and hydrophobic forces played a major role in the binding of 4-nitroaniline and SA. The results of synchronous fluorescence showed the polarity around tryptophan residues was decreased and the hydrophobicity was increased.

You might also be interested in these eBooks
Environmental Protection and Sustainable Development View Preview

Info:

Periodical:

Applied Mechanics and Materials (Volumes 522-524)

Pages:

337-340

DOI:

https://doi.org/10.4028/www.scientific.net/AMM.522-524.337

Citation:

Cite this paper

Online since:

February 2014

Authors:

Yan Qiu Liang*, Ying Zhang

Keywords:

4-Nitroaniline, BSA, Fluorescence Spectroscopy, HSA, Thermodynamic Parameters

Export:

RIS, BibTeX

Price:

Permissions CCC:

Request Permissions

Permissions PLS:

Request Permissions

Сopyright:

© 2014 Trans Tech Publications Ltd. All Rights Reserved

Share:

Citation:

* - Corresponding Author

References

[1] G. H. Lu, X. Yuan and Y. H. Zhao: Environ. Chem. Vol. 19 (2000), p.225.

Google Scholar

[2] D. L. Xi, Y. S. Sun: Environmental Monitoring ( Higher Education press, China 2010).

Google Scholar

[3] A. Papadopoulou, R.J. Green and R.A. Frazier: J. Agric. Food Chem Vol. 53 (2012), p.158.

Google Scholar

[4] C. Dufour, O. Dangles: Biochim. Biophys. Acta Vol. 1721 (2009) p.164.

Google Scholar

[5] G. Colmenarejo: Med. Res. Rev. Vol. 23 (2003) p.275.

Google Scholar

[6] J.H. Tang, F. Luan and X.G. Chen: Bioorg. Med. Chem Vol. 14 (2006) p.3210.

Google Scholar

[7] Y.J. Hu, Y. Liu and R.M. Zhao: J. Biol. Macromol Vol. 37 (2005) p.122.

Google Scholar

[8] E.L. Gelamo, C.H.T.P. Silva, and H. Imasato: Biochim. Biophys. Acta Vol. 1594 (2002) p.84.

Google Scholar

[9] J.R. Lakowicz: Principles of Fluorescence Spectroscopy (Plenum Press，America 1999).

Google Scholar

[10] B. Sengupta, P.K. Sengupta: Biochem. Biophys Res. Commun Vol. 299 (2002) p.400.

Google Scholar

[11] X.M. He, D.C. Carter: Nature Vol. 358 (1992) p.209.

Google Scholar

[12] Y.J. Hu, Y. Liu and J. Wang: J. Pharm. Biomed. Anal Vol. 36 (2004) p.915.

Google Scholar

[13] A. Mallick, S.C. Bera and S. Maiti: Biophys. Chem. Vol. 112 (2004) p.9.

Google Scholar

[14] S. Bi, D. Song, Y. Tian and X. Zhou: Spectrochim. Acta. Part A Vol. 61 (2005) p.629.

Google Scholar

© 2025 Trans Tech Publications Ltd. All rights are reserved, including those for text and data mining, AI training, and similar technologies. For open access content, terms of the Creative Commons licensing CC-BY are applied.
Scientific.Net is a registered trademark of Trans Tech Publications Ltd.