Isolation and Identification of Main Microorganisms in Different Water Systems in Northern Hangzhou, China

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Microorganisms in aquatic ecosystems play an important role in the processes like water purification, nutrition regeneration and biochemical circulation. Some microorganisms are also related to human diseases. To analyze the main microorganisms in the water systems in northern Hangzhou, water was sampled from three different rivers for isolation and identification of microorganisms. The highest diversity and quantity of bacterium was found in the rivers in Banshan industrial district, with an average 127 clones in each plate. For the Beijing-Hangzhou Grand Canal and Longshan reservoir, the number was 20 and 7, respectively. Similar trends were also observed for actinomycetes and molds. CCA analysis showed that the microorganism abundance was mostly related to the NO3-N, TP and TN values of water. Totally, 7 bacterium strains were identified, including Bacillus cereus, Escherichia coli., Klebsiella pneumoniae, and so on, which belong to 7 different genera. In an antibiotics sensitivity analysis, all of the 7 bacteria showed sensitivity to tetracycline.

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445-449

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February 2014

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© 2014 Trans Tech Publications Ltd. All Rights Reserved

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[1] S. J. Binnerup, K. Jensen, N.P. Revsbech, et al. Denitrification, dissimilatory reduction of nitrate to ammonium, and nitrification in a bioturbated estuarine sediment as measured with 15n and microsensor techniques[J]. Applied and Environmental Microbiology, 1992, 58: 303-313.

DOI: 10.1128/aem.58.1.303-313.1992

Google Scholar

[2] R. Sinsabaugh and S. Findlay. Microbial production, enzyme activity, and carbon turnover in surface sediments of the hudson river estuary[J]. Microbial. Ecology, 1995, 30: 127-141.

DOI: 10.1007/bf00172569

Google Scholar

[3] J.L. Pinckney, H.W. Paerl, P. Tester, et al. The role of nutrient loading and eutrophication in estuarine ecology. Environmental Health Perspectives, 2001, 109: 699.

DOI: 10.2307/3454916

Google Scholar

[4] T. Yokokawa and T. Nagata. Linking bacterial community structure to carbon fluxes in marine environments[J]. Journal of Oceanography, 2010, 66: 1-12.

DOI: 10.1007/s10872-010-0001-4

Google Scholar

[5] N. Pimenov, M. Ulyanova, T. Kanapatsky, et al. Microbially mediated methane and sulfur cycling in pockmark sediments of the gdansk basin, baltic sea. Geo-Marine Letters, 2010, 30: 439-448.

DOI: 10.1007/s00367-010-0200-4

Google Scholar

[6] A.E. Bernhard, T. Donn, A.E. Giblin, et al. Loss of diversity of ammonia-oxidizing bacteria correlates with increasing salinity in an estuary system[J]. Environmental Microbiology, 2005, 7(9): 1289-1297.

DOI: 10.1111/j.1462-2920.2005.00808.x

Google Scholar

[7] A. Pernthaler, J. Pernthaler, R. Amann. Fluorescence in situ hybridization and catalyzed reporter deposition for the identification of marine bacteria. Applied and Environmental Microbiology, 2002, 68: 3094.

DOI: 10.1128/aem.68.6.3094-3101.2002

Google Scholar

[8] T.E. Freitag, L. Chang, J.I. Prosser. Changes in the community stucture and activity of betaproteobacterial ammonia-oxidizing sediment bacteria along a freshwater-marine gradient[J]. Environmental Microbiology, 2006, 8(4): 684-696.

DOI: 10.1111/j.1462-2920.2005.00947.x

Google Scholar

[9] D.J. Scala, L.J. Kerkhof. Horizontal heterogeneity of denitrifyingbacterial communities in marine sediments by terminal restriction fragment length polymorphism analysis[J]. Applied and Environmental Microbiology, 2000, 66: (1980).

DOI: 10.1128/aem.66.5.1980-1986.2000

Google Scholar

[10] F.L. Sun, Y.S. Wang, M.L. Wu, et al. Spatial and vertical distribution of bacteria in the Pearl River estuary sediment[J]. African Journal of Biotechnology, 2012, 11(9): 2256-2266.

DOI: 10.5897/ajb11.568

Google Scholar

[11] Environmental Protection Administration of China. 2002. The surface water environmental quality standard of China(GB3838-2002), 2006. (in Chinese).

Google Scholar

[12] The ministry of health of the People's Republic of China. 2006. The standard test methods of drinking water health(GB/T5750-2006), T57502. 2006. (in Chinese).

Google Scholar

[13] J. Zhang, W. Chen. Analysis of the microbial community structure in the Yellow River sanmenxia wetland. Journal of Sanmenxia Polytechnic. 2011, 10(3): 106-108 (in Chinese).

Google Scholar

[14] H. Li, W.S. Guan, E.M. Ouyang, et al. DGGE technique and its application in environmental microbiology. Environmental science and management, 2008, 33(10): 93-96 (in Chinese).

Google Scholar

[15] Y. Jia, C.J. Sun, W.Y. Han, et al. A review of Klebsiella pneumoniae. Journal of microbiology, 2006, 26(5): 75-78 (in Chinese).

Google Scholar