With the rapid development of tourism in“3+5” urban agglomeration, more and more attention is paid to ecological security triggered by tourism wave. Taking Loudi City in urban agglomeration as an example, conduct empirical study on the application of ecological footprint theories and methods on the quantitative evaluation of sustainable development of regional tourism. The results show: tourism ecological footprint per capita of Loudi was 768.9881×10-5hm2 in 2009 in which transportation taking 55.3156% is the maximum, and food consumption taking 39.7622% is the second one. Tourism ecological capacity per capita was 1520.5170×10-5hm2; tourism ecological surplus per capita was 751.5289×10-5hm2 and ecological security degree was 0.5057 which showed a common condition or could be said as a safer condition. However, with the rapid development of tourism, tourism ecological footprint of Loudi City shows an increasing trend year by year. We should coordinate well the relationship between tourism development and ecological environmental protection.