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A Surface Plasmon Resonance Biosensor for the Determination of Salmonella

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Abstract:

A novel and sensitive method has been successfully developed for quantitative detection of invA gene of Salmonella. The surface of sensor chip was modified and then target sequence and probe-2 were induced, which formed a sandwich structure. Under optimal conditions, the SPR-based DNA sensor displayed a linear range of 200 pM - 10 nM and a low detection limit of 200 pM for synthetic target sequence. The excellent sensitivity and high stability of the designed biosensor, make this strategy a promising tool for Salmonella screening.

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Periodical:

Applied Mechanics and Materials (Volumes 568-570)

Pages:

519-522

DOI:

https://doi.org/10.4028/www.scientific.net/AMM.568-570.519

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Online since:

June 2014

Authors:

Yu Rong Yan, Pin Hua Lei, Ye Zhang, Wei Cheng, Shi Jia Ding*

Keywords:

Biosensor, Determination, Salmonella, Surface Plasmon Resonance

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© 2014 Trans Tech Publications Ltd. All Rights Reserved

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