Efficient PEGfusion Combinedoptical Tweezers and Dielectrophoresis

Yoshihiro Mizuta; Kozo Taguchi

doi:10.4028/www.scientific.net/AMM.595.61

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HomeApplied Mechanics and MaterialsApplied Mechanics and Materials Vol. 595Efficient PEGfusion Combinedoptical Tweezers and...

Efficient PEGfusion Combinedoptical Tweezers and Dielectrophoresis

Abstract:

Cell fusionis difficult so that research institutions try to fusion with many methods. For example, method of using polyethylene glycol (PEG) is useful and it mainly use in fusion. However cell fusion efficiency of this method is less. In this paper we suggest efficient fusion of PEG with combining optical tweezers and dielectrophoresis (DEP). Optical tweezers is useful tool in cell manipulation ant it has features of non-invasive and non-contact. Using this technique, we can take target cell from many cells. DEP are known to manipulate cell and form pearl chain by non-uniform electric field. We think DEP lead to efficient cell fusion of PEG because probability of cell adhered by only PEG is less.So we performed firstly take protoplast of red cabbage as specific cell from cells to parallel electrodes by optical tweezers and second, we observed cell-cell fusion by PEG with cell formed pearl chain by DEP. Furthermore we demonstrated using optical tweezers at 980 nm, showed manipulation dates of polymer microspheres, yeast cell and protoplast of red cabbage.

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Periodical:

Applied Mechanics and Materials (Volume 595)

Pages:

61-64

DOI:

https://doi.org/10.4028/www.scientific.net/AMM.595.61

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Online since:

July 2014

Authors:

Yoshihiro Mizuta*, Kozo Taguchi

Keywords:

Dielectrophoresis, Fusion, Optical Tweezers, Protoplast

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