Fabrication of Cell Outer Membrane Mimetic Surfaces on Chitosan Nanoparticles by Polyionic Complex and Template Polymerization

Abstract:

The polyanion of copolymer poly (2-methacryloyloxyethyl phosphorylcholine-co-methacrylic acid) (PMA30) was synthesized by free radical polymerization of 2-methacryloyloxyethyl phosphorylcholine (MPC) and methacrylic acid (MA). The synthesized PMA30 was assembled on chitosan (CS) surfaces formation of chitosan nanoparticles by polyionic complex. The chitosan nanoparticle was prepared using methacrylic acid (MA), 2-methacryloyloxyethyl phosphorylcholine (MPC) by template polymerization. The size distribution and structure properties of chitosan nanoparticles were characterized by scanning electron microscopy (SEM), dynamic light scattering (DLS), transmission electron microscopy (TEM) and zeta potential. These results show that a cell outer membrane mimetic surface was formed on the chitosan nanoparticles, which provides an effective way to improve the biocompatibility of chitosan nanoparticles.

• Periodical: Applied Mechanics and Materials (Volume 618)
• Pages: 335-338
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.618.335
• Online since: August 2014
• Authors: Ming Gong*, Zhu Feng Li, Qi Wang, Kai Li Sheng, Xiao Qin Wang, Yong Kuan Gong
• Keywords: Cell Outer Membrane Mimetic, Chitosan, Nanoparticle, Phosphorylcholine

References

[1] M. Rinaudo, Chitin and chitosan: Properties and applications, Progress in Polymer Science 31 (2006) 603-632.

DOI: 10.1016/j.progpolymsci.2006.06.001

[2] S. Mansouri, J. Fatisson, Z. Miao, Y. Merhi, F.M. Winnik, M. Tabrizian, Silencing Red Blood Cell Recognition toward Anti-A Antibody by Means of Polyelectrolyte Layer-by-Layer Assembly in a Two-Dimensional Model System, Langmuir 25 (2009).

DOI: 10.1021/la9016799

[3] K.F. Ren, Y.X. Wang, J. Ji, Q.K. Lin, J,C. Shen, Construction and deconstruction of PLL/DNA multilayered films for DNA delivery: Effect of ionic strength, Colloids and Surfaces B: Biointerfaces 46 (2005) 63-69.

DOI: 10.1016/j.colsurfb.2005.09.004

[4] A. Reisch, J.C. Voegel, E. Gonthier, G. Decher, B. Senger, P. Schaaf, P.J. Mésini, Polyelectrolyte Multilayers Capped with Polyelectrolytes Bearing Phosphorylcholine and Triethylene Glycol Groups: Parameters Influencing Antifouling Properties, Langmuir 25 (2009).

DOI: 10.1021/la8037846

[5] Y. Hu, X.Q. Jiang, Y. Ding, H.X. Ge, Y.Y. Yuan, C.Z. Yang, Synthesis and characterization of chitosan–poly(acrylic acid) nanoparticles, Biomaterials 23 (2002) 3193-3201.

DOI: 10.1016/s0142-9612(02)00071-6

[6] B. Feng, R.Y. Hong, Y.J. Wu, G.H. Liu, L.H. Zhong, Y. Zheng, J.M. Ding, D G. Wei, Synthesis of monodisperse magnetite nanoparticles via chitosan–poly(acrylic acid) template and their application in MRI, Journal of Alloys and Compounds 473 (2009).

DOI: 10.1016/j.jallcom.2008.05.094

[7] M.R. deMoura, F.A. Aouada, L.H.C. Mattoso, Preparation of chitosan nanoparticles using methacrylic acid, Journal of Colloid and Interface Science 321 (2008) 477-483.

DOI: 10.1016/j.jcis.2008.02.006