Inactivation of Giardia intestinalis by H₂O₂/O₃

Abstract:

The objective of this study was to investigate the effect of H₂O₂/O₃ inactivating Giardia intestinali (G. intestinali) by the fluorescence staining method and vitro excystation. Results suggested that hydroxyl radicals (•OH) was the principal factor during the H₂O₂/O₃ process by testing the effect of t-butyl alcohol (TBA) and concentration change of H₂O₂. The inactivation rate achieved 99.6% when independent addition of O₃ under 1.5 mg·L^-1 with contact time about 10.0 min. While to achieve the same inactivation effect by H₂O₂/O_3, 1.0 mg·L^-1 O₃ ([H₂O₂]/[O₃] molar ratio = 0.8) with 7.0 min was required. The inactivating capability was stronger under acidic condition than in alkaline environment. The effect of turbidity was also found to be significant in synthetic water, when turbidity increasing, the inactivating effect declined. Inactivation rate was improved with a temperature increase from 5 to 35 °C. When dissolved organic matter concentration in the reaction system increased, the competitive reaction between G. intestinal and organics with H₂O₂/O₃ probably took place, thereby reducing the inactivation rate.

• Periodical: Applied Mechanics and Materials (Volumes 675-677)
• Pages: 134-139
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.675-677.134
• Online since: October 2014
• Authors: Shao Feng Li*, Zhi Lin Ran
• Keywords: Giardia intestinalis, H₂O₂/O₃, Inactivation

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