Effect of Bap on the Expression of Cx43 in Leydig Cells

Abstract:

Polycyclic aromatic hydrocarbon (PAHs), espically benzopyrene (BaP),are the most popular pollutants in surface water,and the adverse effects on male reproductive function have aroused more and more attention. The objective of this study is to confirm BaP regulates testosterone (T) in adult rat Leydig cells and then deserve the effect of BaP on Cx43 between Leydig cells, in order to explore whether the influence of Bap on Leydig cells is via its effect on Cx43. Established the primary culture system Leydig cell, Primary cultured Leydig cells were incubated in the different concentrations (1;10;50ug/ml) of BaP, and DMSO control group. After culturing 24h, the expression of Cx43 on cell membrane were investigated with immunofluorescence and the total Cx43 were evaluated by Western blotting.. The results indicate: comparing with the control group, the expression of Cx43 are suppressed by Bap in a dose-response relationship.

• Periodical: Applied Mechanics and Materials (Volumes 675-677)
• Pages: 167-170
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.675-677.167
• Online since: October 2014
• Authors: Huan Li, Yi Zhang, Shu Guang Jin*
• Keywords: Benzopyrene (BaP), Cx43, Rat Leydig Cells

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