Simultaneous Determination of Four Plant Hormones in Soil by Ultrasound-Assisted Ionic Liquid Based Dispersive Liquid-Liquid Microextraction

Abstract:

An ultrasound-assisted ionic liquid based dispersive liquid-liquid microextraction (UA-IL-DLLME) was developed for the determination of four plant hormones (6-benzyladenine (6-BA), kinetin (6-KT), 2, 4-dichlorophenoxy acetic acid (2, 4-D) and uniconazole (UN)) in soil, using high performance liquid chromatography (HPLC)-diode array detection (DAD). Several important parameters including the type and volume of extraction solvent, the volume of disperser solvent, ultrasound time, pH of the solution and salt effect were studied and optimized. Under optimum conditions, the limits of detections (LODs) for the target analytes were in the range of 0.002-0.01 μg g^-1. And satisfactory recoveries of the target analytes in the soil samples were 79.3-96.7 %, with relative standard deviations (RSD, n=5) that ranged from 4.3 to 6.7%.

• Periodical: Applied Mechanics and Materials (Volumes 675-677)
• Pages: 181-184
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.675-677.181
• Online since: October 2014
• Authors: Gui Qi Huang, She Ying Dong, Zhen Yang, Ting Lin Huang
• Keywords: High Performance Liquid Chromatography, Plant Hormones, Soil, Ultrasound Assisted Ionic Liquid Based Dispersive Liquid-Liquid Microextraction

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