Paper Titles

Investigations on Composite Flexural Behaviour with Inclusion of CNT Enhanced Silica Aerogel in Epoxy Nanocomposites
p.179

Synthesis and Application of Carbon Cryogel Beads Using Coconut Husk for Dye Removal
p.183

Effects of the Processing Methods on the Gas Permeability of PLA/Sepiolite Thin Films: Solution Casting versus Thermo Compression
p.187

Fabrication of Poly Caprolactone (PCL) Based Microspheres for Drug Delivery and Tissue Engineering Application
p.191

Fabrication and Characterization of PCL/GE-Based Electrospun Nanofibers for Tissue Engineering and Drug Delivery Application
p.195

Fabrication of BSA Loaded Poly(Caprolactone) (PCL) Microsphere Incorporated Chitosan Scaffolds for Tissues Engineering Application
p.199

Polycaprolactone(PCL)/Chitosan(Cs)-Based Scaffold by Freeze Drying Technique for Tissue Engineering and Drug Delivery Application
p.203

Synthesis of Nano-Polyaniline Using Different Ultrasonic Wave
p.207

Identification of Chemical Compound of Dammar Resin Using Various Solvents with Gas Chromatographic–Mass Spectrometric Method
p.211

HomeApplied Mechanics and MaterialsApplied Mechanics and Materials Vol. 695Fabrication and Characterization of PCL/GE-Based...

Fabrication and Characterization of PCL/GE-Based Electrospun Nanofibers for Tissue Engineering and Drug Delivery Application

Article Preview

Abstract:

Tissue engineering (TE) provides an alternative option to solve limitation of organ and tissue transplantation issues. Fabrication of scaffold is a major challenge because it needs to provide a suitable medium for cell growing and drug delivery which enhances cell transplantation efficiency. The porosity, biodegradability and biocompatibility are the important properties in fabricating a scaffold. Our previous work had found that electrospinning of Polycarprolactone (PCL)/ Gelatin (GE)-based nanofibers in 14% w/v polymer solution in 18kV showed the best results in morphology, average diameters, average pore size and hydrophilicity. Hence, in this project, we are interested to study the relationship between weight ratio of PCL and GE in the same concentration (14%) to the morphology, degradation rate and porosity of nanofibers. Experimental results showed that PCL with 1.2g and GE with 0.2g was able to produce better nanofibers. The sample was then further deployed in drug (tetracycline hydrochloride) loading and it was successfully loaded proven by Energy Dispersive X-Ray (EDX) Spectrum. These nanofibers are predicted to be potentially useful for drug delivery and TE application.

You might also be interested in these eBooks

Advanced Research in Materials and Engineering Applications

Info:

Periodical:

Applied Mechanics and Materials (Volume 695)

Pages:

195-198

DOI:

https://doi.org/10.4028/www.scientific.net/AMM.695.195

Citation:

Cite this paper

Online since:

November 2014

Authors:

Lor Huai Chong, Mim Mim Lim, Naznin Sultana*

Keywords:

Degradation, Drug Loading, Electrospinning, Electrospun Nanofibers, Gelatin (Ge), Polycaprolactone (PCL), Porosity

Export:

RIS, BibTeX

Price:

Permissions CCC:

Request Permissions

Permissions PLS:

Request Permissions

Сopyright:

© 2015 Trans Tech Publications Ltd. All Rights Reserved

Share:

Citation:

* - Corresponding Author

[1] Information on http: /www. organdonor. gov/index. html.

[2] X.M. Peter, J. Elisseeff, Scaffolding In Tissue Engineering, first ed., CRC Press, United State, (2005).

[3] J. Venugopal, S. Low, T.C. Aw, S. Ramakrishna, Interaction of Cells and Nanofiber Scaffolds in Tissue Engineering, Journal of Biomedical Materials Research Part B: Applied Biomaterial. 84B (2008) 34-48.

DOI: 10.1002/jbm.b.30841

[4] N. Sultana and T. H. Khan: Water Absorption and Diffusion Characteristics of Nanohydroxyapatite (nHA) and Poly(hydroxybutyrate-co-hydroxyvalerate-) Based Composite Tissue Engineering Scaffolds and Nonporous Thin Films. J Nanomater, vol. 2013, Article ID 479109, pp.1-8 (2013).

DOI: 10.1155/2013/479109

[5] R. Sinha, G.J. Kim, S. Nie, D.M. Shin, Nanotechnology in cancer therapeutics: bioconjugated nanoparticles for drug delivery, Molecular Cancer Therapeutics. 8 (2006) 1909-(1917).

DOI: 10.1158/1535-7163.mct-06-0141

[6] J. Venugopal, S. Low, T.C. Aw, S. Ramakrishna, Interaction of Cells and Nanofiber Scaffolds in Tissue Engineering, Journal of Biomedical Materials Research Part B: Applied Biomaterial. 84B (2008) 34-48.

DOI: 10.1002/jbm.b.30841

[7] W. Liu, S. Thomopoulos, Y. Xia, Electrospun Nanofibers for Regenerative Medicine.

[8] T.G. Kim, D.S. Lee, G.P. Tae, Controlled protein release from electrospun biodegradable fiber mesh composed of poly(caprolactone) and poly(ethylene oxide), International Journal of Pharmaceutics. 338 (2007) 276–283.

DOI: 10.1016/j.ijpharm.2007.01.040

[9] L.S. Nair, S. Bhattacharya, C.T. Laurencin, Nanotechnology and tissue engineering: the scaffold based approach, Nanotechnol Life Sci. 8 (2008) 1-6.

[10] N. Sultana, T.H. Khan, In Vitro Degradation of PHBV Scaffolds and nHA/PHBV Composite Scaffolds Containing Hydroxyapatite Nanoparticles for Bone Tissue Engineering, Journal of Nanomaterials, 2012 (2012).

DOI: 10.1155/2012/190950

[11] D. Kołbuk, P. Sajkiewicz, K. Maniura-Weber, G. Fortunato, Structure and morphology of electrospun polycaprolactone/gelatine nanofibres, European Polymer Journal. 49 (2013) 2052-(2061).

DOI: 10.1016/j.eurpolymj.2013.04.036

[12] D. Kai, M.P. Prabhakaran, B. Stahl, M. Eblenkamp, E. Wintermantel, S. Ramakrishma, Mechanical properties and in vitro behavior of nanofiber-hydrogel composites for tissue engineering applications, Nanotechnology. 23 (2012).

DOI: 10.1088/0957-4484/23/9/095705

[13] L. Ghasemi-Mobarakeh, M. P. Prabhakaran, M. Morshed, M. H. Nasr-Esfahani, and S. Ramakrishna, Electrospun poly(Ɛ-caprolactone)/gelatin nanofibrous scaffolds for nerve tissue engineering, Biomaterials. 29 (2008) 4532-4539.

DOI: 10.1016/j.biomaterials.2008.08.007

[14] M.P. Prabhakaran, J.R. Venugopal, S. Ramakrishna, Mesenchymal stem cell differentiation to neuronal cells on electrospun nanofibrous substracts for nerve tissue engineering, Biomaterials. 30 (2009) 4996-5003.

DOI: 10.1016/j.biomaterials.2009.05.057

[15] M.A. Woodruff, D.W. Hutmacher, The return of a forgotten polymer-Polycarprolactone in 21st century, Progress in Polymer Science. 35 (2010) 1217-1256.

DOI: 10.1016/j.progpolymsci.2010.04.002

[16] X. Yang, F. Yang, X.F. Walboomers, M. Fan, J.A. Jansen, The performance of dental pulp stem cells on nanofibrous PCL/gelatin/nHA scaffolds, Journal of Biomedical Materials Research Part A. 93A (2010) 247-257.

DOI: 10.1002/jbm.a.32535

[17] B. Feng, H. Tu, H. Yuan, H. Peng, Y. Zhang, Acetic-Acid-Mediated Miscibility toward Electrospinning Homogeneous Composite Nanofibers of GT/PCL, Biomacromolecules. 13 (2012) 3917-3925.

DOI: 10.1021/bm3009389

[18] G. Tronci, A.T. Neffe, B.F. Pierce, A. Lendlein, An entropy–elastic gelatin-based hydrogel system, J. Mater. Chem. 20 (2010) 8875-8884.

DOI: 10.1039/c0jm00883d

[19] D. Gupta, J. Venugopal, M.P. Prabhakaran, V.R.G. Dev, S. Low, A.T. Choon, S. Ramakrishna, Aligned and random nanofibrous substrate for the in vitro culture of Schwann cells for neural tissue engineering, Acta Biomaterialia. 5 (2009) 2560-2569.

DOI: 10.1016/j.actbio.2009.01.039

[20] Y. Zhang, H. Quyang, C.T. Lim, S. Ramakrishna, Z.M. Huang, Electrospinning of Gelatin Fibers and Gelatin/PCL Composite Fibrous Scaffolds, Wiley InterScience. (2004).

DOI: 10.1002/jbm.b.30128

[21] F. Roozbahani, N. Sultana, A. F. Ismail, and Hamed Nouparvar, Effects of Chitosan Alkali Pretreatment on the Preparation of Electrospun PCL/Chitosan Blend Nanofibrous Scaffolds for Tissue Engineering Application, Journal of Nanomaterials. 2013(2013).

DOI: 10.1155/2013/641502