A Rapid and Sensitive Chemiluminescent Immunoassay of Bisphenol a with NSP-SA-NHS-Labeled

Abstract:

A novel chemiluminescence immunoassay (CLIA) of Bisphenol A (BPA) with the acridinium ester of NSP-SA-NHS-labeled has been developed. In this study, BVA and NSP-SA-NHS had been coupled with BSA, the UV spectrum results indicated the conjugates which were successfully synthesized. Basing on these luminescence data the inhibition curve of BPA was established, then the linear arrang of the curve was between 0.4 ng/ml and 5 ng/ml, the 50% inhibitory concentration (IC₅₀) was 2.3ng/ml, the lowest limit of detection was 0.1ng/ml, which showed it’s an efficient and highly sensitive method.

• Periodical: Applied Mechanics and Materials (Volume 707)
• Pages: 7-11
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.707.7
• Online since: December 2014
• Authors: Fan Fan Yang, Li Xin Zhu, Long Xu, Ren Rong Liu*, Yan Fan, Wei Meng
• Keywords: Bisphenol A, Direct Competition CLIA Method, NSP-Sa-NHS-Labeled

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