Applied Mechanics and Materials Vol. 707

Abstract: The MeGolS5 promoter fragment (1492 bp) was amplified from the genomic DNA of Manihot esculenta Crantz by inverse polymerase chain reaction. Promoter sequence analysis by PLACE and PlantCARE showed that the cloned fragment contained several putative cis-elements, such as abscisic acid response element (ABRE), heat shock elements (HSE), as well as TATA-Box and CAAT-Box. The expression prfile of MeGolS5 shows that the gene is induced by several abiotic stresses, such as salt, drought, H₂O₂ and ABA.

Abstract: Analgin is a widely used medicine for its significant effect, convenience and low price. At present, there still exist some problems concerning the production process. The purpose of this work is to investigate present situation of analgin preparation, the disadvantages and existing problems, to discuss the possibility to optimize the process. An efficient approach to improve the process is to remove 4-aminoantipyrine sulfate related materials by extraction, which simplified the post-processing, greatly increased analgin crystallization rate, and reduced mother liquor quantity.

Abstract: In order to induce embryo from callus and set up the integral somatic embryo induction system from monoclonal cell, the differences of cell morphology and structure from different types of alfalfa callus were observed and compared by quick section and microscopic examination. The results show that giant callus cells were larger, elongated and yellow-white; loose callus cells were smaller, spherical, soft and yellow-green; hard-type callus cells were round, hard and dark green. The cell volume of giant callus was 4.5 times more than loose callus cells and 9 times more than hard-type callus cells. The biggest change of vacuole number and form were giant callus cells, which had 48%cells of 5-8 big vacuoles. Loose callus cells had 89%cells of 2-4 small vacuoles and hard-type callus cells had 97% cells with one large central vacuole. Loose callus cells had more chloroplast, which were 4.65 times more than giant callus cells. The chloroplast of hard-type callus cells was gathered into groups, which had 3-5 chloroplasts in it. The most nucleuses of giant callus cells and loose callus cells were located in the central of cell and 96.8% nucleus of hard-type callus cells were located on the edge. In hard-type callus cells there were different number of rings, thread and textured ducts. In short, there were lower cell differentiation and clearer vacuolization in giant callus, and high degree of differentiation and tissue aging in hard-type callus. The loose callus was undifferentiated, was lower on vacuolization and apparent on characteristics of embryonic callus, so that it was more suitable for induction of somatic embryos.

Abstract: The paper discussed the extraction process of rubusoside from Rubus suavissimus using a new technique named cellulase pretreatment approach. First, the parameters of cellulose which generate the optimized condition for the extraction of rubusoside from Rubus suavissimus were identified. Under the most optimal condition, the extraction efficiency is maximal. The parameters include pH value,temperature and enzyme-substrate ratio. Second, single factor experiment and an orthogonal test then could be conducted to obtain the optimized condition for the extraction process with the assistances of cellulose. The parameters under the optimized condition were determined as following: ethanol as solvent, 1.2% cellulose, 40°C, pH 4.8 and duration 25 minutes. The result shows that the extraction efficiency of rubusoside is 10.81%.

Abstract: Microalgae protein hydrolysates (MPH) were obtained by enzymatic hydrolysis of defatted microalgae meal using neutral protease. The protein recovery, degree of hydrolysis, and the antioxidant activities of the hydrolysates were investigated. The results demonstrated that hydrolysates prepared by neutral protease at 50 °C for 4 h exhibited the strongest antioxidant activity. Under these conditions, the 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl radical scavenging activity and the reducing power of the hydrolysates were 68.3%, 50.8% and 1.303, respectively.

Abstract: Calcium sulfate/hydroxyl apatite whiskers composite is possible to be used as bone graft substitute, for its biocompatibility, controllable degradation, and suitable mechanical properties. In this study, calcium sulfate/hydroxyl apatite whisker composites were fabricated and characterized. The characteristics of the composites were assessed by means of scanning electron microscopy (SEM), X-ray diffraction (XRD) and immersing testing techniques. The composites prepared in this article have been confirmed to be ideally used as biodegradable implants.

Abstract: Mg and its alloys draw more and more attentions to be used as biodegradable medical materials. But the rapid degradation of those materials limits their clinical applications. In this article, a phosphate coating was prepared on AZ31B magnesium alloy to improve its anti-corrosion property. Morphology, composition and corrosion resistance of the coating were studied. The SEM research showed that the coating that composed of rod-like phosphates with small amount of random distributed micro-voids was approximately10-20μm in thickness. The XRD analysis indicated that the coating was mainly composed of MgO, Mg₃(PO₄)₂and Zn₃(PO₄)₂·xH₂O. Electrochemical test showed the phosphate treatment could significant improve the corrosion resistance of AZ31B.

Abstract: Magnesium alloy has been considered as a revolutionary biodegradable material, but the fast degradation limits its development. A permanganate conversion coating for AZ31B magnesium alloy prepared, in order to improve its anti-corrosion property. The morphology and composition of the coating were characterized with scanning electron microscopy (SEM), X-ray diffraction (XRD) and energy dispersion spectroscopy (EDS), which showed that the coating was 40-170μm in thickness, with net-like micro-cracks, and was tightly combined with AZ31B magnesium alloy matrix. The main compositions of the coating were Mg and a series of oxides of Mn. Observed by the tests of electrochemical and immersion,the corrosion resistance of AZ31B magnesium alloy can be improved significantly through the permanganate conversion treatment

Abstract: In this paper, samples were digested by high pressure digestion pots, reducing the loss of trace elements during the digestion process. The content of trace elements in Natural Huazi Mushroom such as Ca was determined by ICP-AES, and compared with the results of wet method, the two methods showed no obvious differences. High pressure nitrifying pot method relative standard deviation of Ca element is 3.36%; Wet method to determine the relative standard deviation of Ca elementt is 7.08%. Visible high pressure nitrifying pot method and treatment of samples of precision of the measuring precision and wet processing samples are relatively good, the measuring precision of the former better. The method was suitable for the determination of trace elements in edible fungi with the advantages of being simple, rapid, sensitive, stable and accurate etc., and the results were satisfactory.

Abstract: Iron is indispensable to make hemoglobin and other iron material elements, is also necessary to exchange and oxygen in the blood of an element, many organisms in REDOX system cannot leave it. Most of the iron distribution in special cells in the body. Using the high pressure nitrifying pot method and wet method and treatment of samples were determined in Comb Mushroom samples Fe element content, the determination results are shown in table 2. High pressure nitrifying pot method relative standard deviation of Fe element is 3.689%; Wet method to determine the relative standard deviation of Fe elementt is 5.473%. Visible high pressure nitrifying pot method and treatment of samples of precision of the measuring precision and wet processing samples are relatively good, the measuring precision of the former better. By comparison, high pressure nitrifying pot method specific humidity method to deal with the sample good measuring precision, higher accuracy, and the measurement process to save time.