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Screening of Acid and Bile Resistant Bifidobacterium bifidum Strains

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Abstract:

This study was conducted to screen the acid and bile resistant Bifidobacterium bifidum strains through repeatedly domesticating. In acid-resistant domestications, low pH tolerance Bifidobacterium bifidum single colonies were obtained by gradually lowering the pH of the liquid TPY basic medium. And then the achieved acid-resistant strains were domesticated through gradually increasing the concentration of bile salt in milk medium. Each time acid and bile resistant single colony was selected after the domestication. The results showed the domesticated Bifidobacterium bifidum strain presents high acid and bile resistant capabilities; morphologic observation and sugar ferment experiments results indicate the domesticated stains did not have any unique mutations. This research showed practical application potential development viable maintenance of Bifidobacterium bifidum products.

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Periodical:

Applied Mechanics and Materials (Volumes 713-715)

Pages:

2855-2858

DOI:

https://doi.org/10.4028/www.scientific.net/AMM.713-715.2855

Citation:

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Online since:

January 2015

Authors:

Hong Ling Wang*, Dan Dan Liu, Shi Wen Jiang, Ting Ru Wu, Yan Lv

Keywords:

Acid-Resistant, Bifidobacterium bifidum, Bile-Resistant, Domestication

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© 2015 Trans Tech Publications Ltd. All Rights Reserved

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