Immobilization of Papain on Flexible Magnetic Nanoparticles

Lian Tan; Lan Qian Li; Jing Dong; Zu Lan Liu; Yi Ping Liu; Ming Lu

doi:10.4028/www.scientific.net/AMM.723.511

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HomeApplied Mechanics and MaterialsApplied Mechanics and Materials Vol. 723Immobilization of Papain on Flexible Magnetic...

Immobilization of Papain on Flexible Magnetic Nanoparticles

Abstract:

Flexible magnetic nanoparticles decorated with dialdehyde starch (DAS) were developed and used as a novel enzyme support for the covalent conjugation of papain. The analyses of Fourier transform infrared (FTIR) spectroscopy confirmed the preparation of magnetic particles with flexible long molecular chains on their surfaces and conjugation of papain with the Fe3O4-DAS nanoparticles. Considering that the immobilized papain was found to exhibit better tolerance to the variations of temperature and medium pH, an advantage of easy to magnetic separation and lack of negative effect on biological activity, the kind of flexible magnetic bioconjugate support should be a good immobilized enzyme carrier, and has potential application in textile, leather, food industries.

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Periodical:

Applied Mechanics and Materials (Volume 723)

Pages:

511-514

DOI:

https://doi.org/10.4028/www.scientific.net/AMM.723.511

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Online since:

January 2015

Authors:

Lian Tan, Lan Qian Li, Jing Dong, Zu Lan Liu, Yi Ping Liu, Ming Lu*

Keywords:

Flexible Decoration, Immobilization, Magnetic Particles, Papain

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* - Corresponding Author

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