Effect of Natural Cyclodextrins on Cell Growth, Activity and Permeability of Arthrobacter simplex

Abstract:

In this study, the biotransformation which from cortisone acetate (CA) to prednisone acetate (PA) by Arthrobacter simplex (ASP) was selected as a model system for evaluating the effects of natural cyclodextrins (α-CD, β-CD and γ-CD, referred as CDs) on cell growth, dehydrogenase activity, permeability of Arthrobacter simplex. The results showed that the cavity size of cyclodextrins on ASP cell growth were quite different, the with smaller cavity significantly inhibited ASP growth. CDs reduced the dehydrogenase activity of ASP and increased the cell permeability. Initial conversion rate of cells after treated by CDs were higher than the control cells. The study provided basic datas for the application of cyclodextrin in the steroid biotransformation.

• Periodical: Applied Mechanics and Materials (Volume 730)
• Pages: 249-253
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.730.249
• Online since: January 2015
• Authors: Qian Gao, Yan Bing Shen, Wei Huang, Min Wang
• Keywords: Arthrobacter simplex, Cell Permeability, Cyclodextrin, Dehydrogenase

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