UPLC-ESI-MS/MS Method for Determination of Recombinant Antihypertensive Peptide in Rat Plasma

Abstract:

The focus of the study was to establish an UPLC-ESI-MS/MS method for the qualitative and quantitative determination of VLPVPR in rat plasma. The method was as follows: Protein impurities in the rat plasma samples were precipitated with methanol; A Waters Acquity BEH C18 column (100 mm×2.1 mm, 1.7 μm) was adopted; the column temperature was 25°C; the mobile phase consisted of methanol-water with gradient elution at the steady flow rate of 0.3 mL·min-1; the detection wavelength was 202 nm. Mass spectrometry applied selected ion monitoring mode with m/z 255 (quantitative ion) and m/z 169 (qualitative ion). It was found that excellent linear relationship was obtained from the range of 10~200 ng·mL-1 (r=0.9991), the limit determination (LOD) of VLPVPR was 1.8 ng·mL-1, the recovery rate was 96.33%~100.76%, The inter and intre-day RSD were less than 7%. We thus conclude that a rapid and sensitive UPLC-ESI-MS/MS method was developed and validated for the determination of recombinant antihypertensive peptide VLPVPR in rat plasma. It can be applied to evaluate the pharmacokinetics of VLPVPR.

• Periodical: Applied Mechanics and Materials (Volume 730)
• Pages: 254-259
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.730.254
• Online since: January 2015
• Authors: Yan Li Cong, Shen Li, Bo Chen, Hai Yan Sun, Dong Liu, Shu Fen Cui
• Keywords: Rat Plasma, Recombinant Antihypertensive Peptide VLPVPR, UPLC-ESI-MS/MS

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