Preparation and Detection of the Resolution and Number of Theoretical Plates for High Purity Steviosides

Abstract:

Macroporous resin was the most common method for the separation and purification of steviosides. But the purity was only 90% influenced by less selectivity of the resin. And Rebaudioside A could not be separated from other steviosides at the same time. Purity of total glycosides could be up to 99% and Rebaudioside A could be enriched efficiently by section desorption after one-dimensional chromatography of macroprous resins. The content of Rebaudioside A increased from 39.46% to 63.70% gradually in each desorption solution of the 4 resin columns. Moreover, the resolution between Rebaudioside A, Rebaudioside C and stevioside, and the number of theoretical plates for them were detected meanwhile. These results could provide theoretical foundation for the separation of high purity steviosides and enrichment of Rebaudioside A.

• Periodical: Applied Mechanics and Materials (Volume 733)
• Pages: 270-275
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.733.270
• Online since: February 2015
• Authors: Jing Bin Liu*, An Jun Liu, Lin Jie Zhang, Yong Liang Zhao, Yin Zhi Xie
• Keywords: Chromatography, Macroporous Resin, Reb-A, Stevioside

References

[1] Madan S, Ahmad S, Singh GN, et al. Stevia rebaudiana (Bert. ) Beitoni-A Review, INJPR, Commun. 1 (2010), pp.267-286.

[2] Rensheng Xu. Chemistry of natural product, Beijing: Science Press, 2004, pp.351-377.

[3] Zhihua Bao. Natural sweetener- stevia glycoside, J. Toothpaste Industry, Commun. 1(1999), pp.34-35.

[4] V.N. Curdoso,E. Muramoto, C.H. Mesquitu, et al. Pharmacokinetic Studies of Stevioside and its Metabolites, J. Nuclear Medicine & Biology, Commun. 3(1996), pp.97-100.

DOI: 10.1016/0969-8051(95)02072-1

[5] Varanuj Chatsudthipong, Chatchai Muanprasat. Stevioside and related compounds: Therapeutic benefits beyond sweetness, J. Pharmacology & Therapeutics, Commun. 121 (2009), pp.41-54.

DOI: 10.1016/j.pharmthera.2008.09.007

[6] Jan M.C. Geuns. Molecules of Interest Stevioside, J. Phytochemistry, Commun, 64 (2003), pp.913-921.

[7] Shanwang Li. Method purification of steviosides by two-step ion exchange, China Patent: ZL1011113. 5 (2009).

[8] Giovanetto R H. Method for the recovery of steviosides from plant raw material, U.S. Patent 4892938, (1990).

[9] Xinghua Zhao, Quanxiang Zhang, Xiaowei Yuan, et al. Purification technology of steviol glycosides by macroporous adsorption resin, Chinese traditional and herbal drugs, Commun. 43(2012), p.1971-(1974).

[10] Tianhong Chen, Yang Zhang, Xiaohang Liu. Research of selectivity adsorption of high polymer sdsorbent for steviol glycosides in leaves of stevia, Science of China(B), Commun. 28(1998), pp.460-465.

[11] Tianhong Chen, Yang Zhang, Zuoqing Shi, et al. Studies on the adsorption and desorption behavior of PYR resin for stevia glycosides, J. Ion exchange and adsorption, Commun, 14(1995), pp.521-525.

[12] B. Vander Bruggena, M. Manttari, M. NySTromb. Drawbacks of applying nanofiltration and how to avoid them: A review, J. Separation and Purification Technology, Commun, 63 (2008), pp.251-263.

DOI: 10.1016/j.seppur.2008.05.010

[13] Shi Qiu Zhang, Ashwani Kumar, Oleh Kutowy. Membrane-based separation scheme for processing sweeteners from Stevia leaves, Food Research International, Commun, 33(2000), pp.617-620.

DOI: 10.1016/s0963-9969(00)00098-3

[14] Yongliang Zhao, Xiao Han, Jingbin Liu, et al. Research of using membrane separation technology to improve traditional sreviosides producting technics, Guangdong chem. Commun, 1 (2010), pp.40-41.

[15] Lingyun Zhang, Huanxin Xu, Zuozhen Liu, et al. Isolation and purification of Rebaoudioside-A by Medium Pressure Preparative Liquid Chromatography, Science and Technology of Food Industry, Commun, 19 (2013), pp.216-224.

[16] Chao Liu, Laisheng Li, Lili Xu, et al. Separation and identification of stevioside and rebaudiodside A in stevia by HPLC, Analysis laboratory, Commun. 26 (2007), pp.23-26.

[17] Jian Shi. Modern column chromatographic analysis, Shanghai: Shanghai Scientific and Technical Literature Publishing House, 2002, pp.6-22.

[18] Xuerong Zhang, Yihe Lu: Technology of medicine separation and purification, Beijing Chemical Industry Press, 2005, pp.106-107.