Optical Microarray Grating Biosensors

Abstract:

An optical biosensor based on a grating to be utilized for the detection of DNA target molecules was fabricated by photolithographic techniques. The sensor surface implements a grating to create a low effective refractive index platform via the combination of Si₃N₄ and SiO₂ which allows the detection via changes of the reflectivity spectra. The active surface carried a layer of probe biomolecules for specific binding of the target DNA. Immobilization of the probe molecules was carried out via streptavidin using biotin modified ssDNA complementary to the target ssDNA. When molecules attached to the surface of the device, the position of the reflectance spectrum shifted due to the change of the optical path of light that is coupled into the grating structure. The extent of the wavelength shift of the peaks could be used to quantify the amount of materials bound to the sensor surface thereby allowing detection of the surface modifications as well as the quantification of the DNA analyte. The advantages of this device are that it works with a small sample volumes (few microlitres), are integratable in micro array type of setups and can be used at room temperature.

• Periodical: Applied Mechanics and Materials (Volume 804)
• Pages: 155-158
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.804.155
• Online since: October 2015
• Authors: Naphat Chathirat*, Charndet Hruanun, Amporn Poyai
• Keywords: DNA Complementary, Microarrays Grating, Photolithography

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