Predicting the UV Spectrum of Single Stranded DNA

Naphat Chathirat

doi:10.4028/www.scientific.net/AMM.804.163

Paper Titles

Effects of the Sintering Temperature on Structural and Bioactivity in BaFe₁₂O₁₉-P₂O₅-CaO-Na₂O Bioactive Glass Ceramic
p.147

Fabrication of Polycaprolactone/Centella asiatica Extract Biopolymer Nanofiber by Electrospinning
p.151

Optical Microarray Grating Biosensors
p.155

Photolithographic Process for Creating Micro-Grating on Silicon Substrate for Biosensor Application
p.159

Predicting the UV Spectrum of Single Stranded DNA
p.163

Preparation of 2 wt% ZnFe₂O₄/HAp Ceramic for Future Biomedical Applications
p.167

Processing of Elastic Silk Sericin/Gelatin Composite Film for Future Cosmetic Applications
p.171

Synthesis of Diamond-Like-Carbon Films as the Coating Prototype for Biomedical Materials
p.175

A Study of the Preparation of Silk Sericin/Chitosan Composite Film for Future Wound Dressing Applications
p.179

HomeApplied Mechanics and MaterialsApplied Mechanics and Materials Vol. 804Predicting the UV Spectrum of Single Stranded DNA

Predicting the UV Spectrum of Single Stranded DNA

Abstract:

A model was developed to predict the UV absorbance spectra and thus concentration of single stranded DNA (ssDNA) samples. The model was developed from UV absorbance spectra of ssDNA oligodeoxynucleotides determined at different concentrations. The model, which would predict the concentration of ssDNA from the A₂₆₀ value, is shown to predict absorbance spectra of ssDNA as shown when compared to the experimental result.

You might also be interested in these eBooks

Innovative Materials and Technology for Sustainable Development of Society

View Preview

Info:

Periodical:

Applied Mechanics and Materials (Volume 804)

Pages:

163-166

DOI:

https://doi.org/10.4028/www.scientific.net/AMM.804.163

Citation:

Cite this paper

Online since:

October 2015

Authors:

Naphat Chathirat*

Keywords:

DNA Prediction, Oligodeoxynucleotide, Uv Spectrum

Export:

RIS, BibTeX

Price:

Permissions CCC:

Request Permissions

Permissions PLS:

Request Permissions

Сopyright:

Citation:

* - Corresponding Author

References

[1] C.R. Cantor, M.M. Warshaw, H. Shapiro, Oligonucleotide interaction. III. Circular dichroism studies of the conformation of deoxyoligonucleotides, Biopolymers. 9 (1970) 1059-1077.

DOI: 10.1002/bip.1970.360090909

Google Scholar

[2] G. Felsenfeld, S.Z. Hirschman, A neighbor-interaction analysis of the hypochromism and spectra of DNA, J. Mol. Biol. 13 (1965) 407-427.

DOI: 10.1016/s0022-2836(65)80106-1

Google Scholar

[3] Chunyu Qiao, Shuyun Bi, Ying Sun, Daqian Song, Hanqi Zhang, Weihong Zhou, Study of interactions of anthraquinones with DNA using ethidium bromide as a fluorescence probe, Spectrochimica Acta Part A. 70, (2008) 136-143.

DOI: 10.1016/j.saa.2007.07.038

Google Scholar

[4] Langer R, Perspective: Drug delivery-drugs on target, Science, 293, (2001) 58-59.

Google Scholar

[5] Weyand E. H, Wu Y, Patel S, Taylor B. B, Mauro D. M, Urinary excretion and DNA binding of coal tar components in B6C3F1 mice following ingestion, Chem Res Toxicol. 4, (1991) 466-473.

DOI: 10.1021/tx00022a011

Google Scholar

[6] Singh M. P, Joseph T, Kumar S, Bathini Y, Lown JW, Synthesis and sequence-specific DNA binding of a topoisomerase inhibitory analog of Hoechst 33258 designed for altered base and sequence recognition, Chem Res Toxicol. 5, (1992) 597-607.

DOI: 10.1021/tx00029a003

Google Scholar

[7] Kelle S. O, Orellana G, Barton J. K, Luminescence quenching by DNA-bound viologens: effect of reactant identity on efficiency and dynamics of electron transfer in DNA, J. Photochem Photobiol B, 58, (2000) 72-79.

DOI: 10.1016/s1011-1344(00)00106-8

Google Scholar

[8] Stout D. L, Becker F. F, Fluorometric quantitation of single-stranded DNA: A method applicable to the technique of alkaline elution, Anal. Biochem. 127 (1982) 302-307.

DOI: 10.1016/0003-2697(82)90177-4

Google Scholar

[9] Gary Kallansrud, Brian Ward, A comparison of measured and calculated single- and double- stranded oligodeoxynucleotide extinction coefficients, Analytical biochemistry, 236 (1996) 134-138.

DOI: 10.1006/abio.1996.0141

Google Scholar

[10] Daxhelet G. A, Coene M. M, Hoet P. P, Cocito C. G, Spectrofluorometry of dyes with DNAs of different base composition and conformation, Anal. Biochem, 179, (1989) 401-403.

DOI: 10.1016/0003-2697(89)90152-8

Google Scholar

[11] Le Pecq, J. B, Use of ethidium bromide for separation and nucleic acids of various conformational forms and measurement of their associated enzymes. In methods of biochemical analysis, John Wiley & Sons, New York, vol. 20 (1971) 41-86.

DOI: 10.1002/9780470110393.ch2

Google Scholar

[12] Wallace R. B, Miyada C. G, Oligonuclotide probes for the screening of recombinant DNA libraries. In methods of enzymology, Academic Press, San Diego, vol. 152 (1987) 432-442.

DOI: 10.1016/0076-6879(87)52050-x

Google Scholar