The Establishment of Real-Time PCR Detection System of Aspergillus Niger in Peanut

Chu Shu Zhang; Jie Sun; Li Na Yu; Jie Bi; Wei Wei Zhao

doi:10.4028/www.scientific.net/AMM.835.333

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The Establishment of Real-Time PCR Detection System of Aspergillus Niger in Peanut
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HomeApplied Mechanics and MaterialsApplied Mechanics and Materials Vol. 835The Establishment of Real-Time PCR Detection...

The Establishment of Real-Time PCR Detection System of Aspergillus Niger in Peanut

Abstract:

In this study, a set of real-time PCR primers for the detection of aflatoxin in peanut was successfully designed according to the AFLR gene of Aspergillus niger. Upstream primer was 5'-AACCTGATGACGACTGATAT-3'; Downstream primer was 5'-AGCACCTTGAGAACGATAA-3'; The real-time PCR detection system established in this study had good specificity, and the sensitivity of DNA detection can reach 3×10^-13ng/ul. The correlation coefficient between the contents of aflatoxin in peanut samples and PCR Ct of Real-time was-0.718, and the correlation coefficient was significantly negative.

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Periodical:

Applied Mechanics and Materials (Volume 835)

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333-337

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https://doi.org/10.4028/www.scientific.net/AMM.835.333

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Online since:

May 2016

Authors:

Chu Shu Zhang*, Jie Sun, Li Na Yu, Jie Bi, Wei Wei Zhao

Keywords:

Aspergillus niger, Detection System, Peanut, Real-Time PCR

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