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Biodesulfurization of Dibenzothiophene by a Newly Isolated Agrobacterium tumefaciens LSU20

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Abstract:

Organic sulfur compound of fossil fuel are too resistant to be removed by the conventional desulfurization processes. This study aimed to investigate the best growth conditions of Agrobacterium tumefaciens strain LSU20 on desulfurized of dibenzothiophene (DBT) compound in the n-tetradecane as model of oil. The experiments were performed with the medium two-phase system, aqueous phase: mineral salt sulfur free (MSSF) medium and the oil phase: n-tetradecane containing 200 ppm of DBT in the ratio of oil/water (1: 5). The culture of LSU20 that has been aged 4 days (OD660 5) of 0.1 ml inoculated in a test tube containing 5 mL of MSSF medium and 1 ml model of petroleum, grown at temperature variations incubation as follows: 33°C, 37°C, 41°C, 45°C, and 49°C; variations in the initial pH of medium: pH 5.0, 5.5, 6.0, 6.5, 7.0, 7.5, and 8.0; and variations of carbon sources such as glucose, sucrose, glycerol and citric acid. The experiments were conducted using a water bath shaker at 150 rpm for 96 hours of incubation. The results showed that the highest rate of degradation of DBT by LSU20 occurs at a temperature of 37°C, media pH of 7 and glucose as the carbon source, ie with the growth rate reached 0.91 (OD660) and DBT compounds degraded until 76.9% (w/v).

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Periodical:

Applied Mechanics and Materials (Volume 855)

Pages:

143-149

DOI:

https://doi.org/10.4028/www.scientific.net/AMM.855.143

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Online since:

October 2016

Authors:

Dr.Ida Bagus Wayan Gunam*, Muhammad Iqbal, I Wayan Arnata, Nyoman Semadi Antara, Anak Agung Made Dewi Anggreni, Yohanes Setiyo, Ida Bagus Putu Gunadnya

Keywords:

Biodesulfurization, Dibenzothiophene, Fossil Fuel, Growth Condition, LSU20 Strain

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