Suitable Concentration of ZnO Nanoparticles Enhanced Biomaterial Synthesis and Plant Cell Protection

Abstract:

Zinc oxide nanoparticles (ZnO NPs) has been reported on toxicity effects on environmental organisms. However, Zn is a plant essential microelement which is involved in various physiological functions. In this study, MS medium, adding 0, 10 and 20 mgL^-1 ZnO NPs were used for culturing wild type (WT) and transgenic expressing PAP1 under tissue culture condition. Effects of ZnO NPs concentrations on the accumulations of total soluble sugar (TSS) and flavonoid biomaterials in WT and transgenic plants were analysis. Membrane stability of each plant was assayed by lipid peroxidation measurement. Malondialdehyde, a byproduct of the membrane lipid peroxidation, was presented. The result showed that both WT and transgenic grown in medium, adding ZnO NPs enhanced the accumulations of TSS and flavonoids. All of PAP1 transgenic lines had a better response under ZnO NPs concentration and elevated higher biomaterials than WT did. Medium adding 20 mgL^-1 ZnO NPs affected the highest accumulation of both biomaterials especially in transgenic plant. Higher accumulation of biomaterials is presented in treatment adding ZnO NPs and plant membrane stability of this treatment is better than that of medium without ZnO NPs. However, medium with 10 mgL^-1 ZnO NPs showed the proper result of plant membrane stability.

• Periodical: Applied Mechanics and Materials (Volume 866)
• Pages: 21-24
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.866.21
• Online since: June 2017
• Authors: Wilailack Chayaprasert, Kanokporn Sompornpailin*
• Keywords: Biomaterials, Cell Protection, Flavonoids, Nanoparticles, PAP1, Zinc, ZNO

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