Study on the Taqman Real-Time PCR to the Detection and Quantification of Rhizoctonia solani AG-3 of Tobacco Target Spot

Abstract:

Rhizoctonia solani Kühn is the causal pathogen of tobacco target spot, a major soilborne fungal pathogen that severely impairs yield and quality of tobacco worldwide. It is difficult to identify and quality the pathogen in plant and soil using conventional methods. In the study we developed a real-time, quantification polymerase chain reaction (QPCR) assay to detect and quantify R. solani AG-3 DNA from infected tobacco tissue and soil. Using a specific primer pair based on the internal transcribed spacer region of the fungal DNA sequence, R. solani AG-3 DNA at quantities as low as 100 fg of purified pathogen DNA could be successfully detect.

• Periodical: Advanced Materials Research (Volumes 1010-1012)
• Pages: 80-83
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.1010-1012.80
• Online since: August 2014
• Authors: Yan Qin Zhao, Yuan Hua Wu*, Xiu Xiang Zhao, Meng Nan An, Jian Guang Chen
• Keywords: Detection, Quantificatinon, Real-Time PCR, Rhizoctonia solani, Tobacco Target Spot

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