The Effect of Oxygen Precursor Concentration to the Iron Oxide Nanoparticles Properties for Lateral Flow Immunoassay Application

Mohamad Nor Noorhashimah; Khairunisak Abdul Razak; Rahmah Noordin

doi:10.4028/www.scientific.net/AMR.1024.277

Paper Titles

Effect of Different Thickness of Core Layer on Tensile Properties of Laminated Natural Rubber Latex Film
p.259

Utilization of Sludge from Sidoarjo Mudflow for Zeolite Synthesis
p.263

Effect of Needle Punching Direction on Nonwoven Fiber Mat to the Mechanical Properties of Kenaf Reinforced Epoxy Composites Produced by Vacuum Assisted Resin Transfer Molding
p.267

Development of Silver-Coated Gold Nanoparticles and its Conjugation for Labeling on Lateral Flow Immunoassay
p.273

The Effect of Oxygen Precursor Concentration to the Iron Oxide Nanoparticles Properties for Lateral Flow Immunoassay Application
p.277

Crytallization and Properties of SiO₂-Al₂O₃-K₂O-ZrO₂ Glass-Ceramics for Dental Applications
p.281

The Design of Elastin-Like Polypeptides (ELPs) as a Nanoparticulate-Based Therapeutic and Diagnostic Imaging Agent
p.287

Loading and Unloading Properties of Encapsulated Methylene Blue in Silica Nanoparticles for Photodynamic Applications
p.292

Poly (Vinyl Alcohol) in Fabrication of PLA Micro- and Nanoparticles Using Emulsion and Solvent Evaporation Technique
p.296

HomeAdvanced Materials ResearchAdvanced Materials Research Vol. 1024The Effect of Oxygen Precursor Concentration to...

The Effect of Oxygen Precursor Concentration to the Iron Oxide Nanoparticles Properties for Lateral Flow Immunoassay Application

Abstract:

In this study, the influence of hydrogen peroxide (H₂O₂) concentration to the iron oxide nanoparticles (IONPs) properties prepared using the precipitation method was investigated. The H₂O₂ concentration was varied from 0.85 M to 5.1 M. The concentration of H₂O₂ influenced the crystallinity and the growth rate of the IONPs precipitates. Increasing the concentration of H₂O₂ increased the crystallinity and expedited the growth rate of IONPs. The optimum concentration of H₂O₂ was 1.7 M. From the transmission electron microscopy (TEM) images, the size of IONPs obtained was ~14 nm and the X-ray diffraction (XRD) spectra showed the presence of spinel cubic lattice of maghemite (γ-Fe₂O₃). The magnetic measurement of IONPs using vibrating sample magnetometer (VSM) was showed that the IONPs exhibited superparamagnetic properties. Furthermore, the electrostatic repulsion using percloric acid (HClO₄) and steric stabilization using silane polyethelene glycol (SiPEG) were created surround IONPs in order to obtain a stable colloidal IONPs for the conjugation process. The stable IONPs were then conjugated to the antibody and tested in the lateral flow immunoassay as the labelling agent.

You might also be interested in these eBooks

Advancement of Materials and Nanotechnology III

View Preview

Info:

Periodical:

Advanced Materials Research (Volume 1024)

Pages:

277-280

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.1024.277

Citation:

Cite this paper

Online since:

August 2014

Authors:

Mohamad Nor Noorhashimah*, Khairunisak Abdul Razak, Rahmah Noordin

Keywords:

Iron Oxide Conjugated Antibody, Iron Oxide Nanoparticles, Lateral Flow Immunoassay

Export:

RIS, BibTeX

Price:

Permissions CCC:

Request Permissions

Permissions PLS:

Request Permissions

Сopyright:

Citation:

* - Corresponding Author

References

[1] F. -H. Wang, T. Yoshitake, D. -K. Kim, M. Muhammed, B. Bjelke and J. Kehr: J. Nanopart. Res. Vol. 5 (2003), p.137.

Google Scholar

[2] Tao, K.,H. Dou, and K. Sun: Colloids and Surface A. Vol. 320 (2008), pp.115-122.

Google Scholar

[3] Jing, J., Zhang, Y., Liang, J., Zhang, Q., Bryant, E., Avendano, C., Colvin, V., Wang, Y., Li, W. and Yu, W: J. Nanopart. Vol. 14 (2012), pp.1-8.

Google Scholar

[4] Alibeigi, S. and M.R. Vaezi: Chemical Engineering & Technology. Vol. 31 (2008), pp.1591-1596.

Google Scholar

[5] Chou. S., Liao. Chi., Perng. S. H., Chang. S. H: Chemosphere. Vol. 54 (2004), pp.859-866.

Google Scholar

[6] Noorhashimah M. N, Nor Dyana Z., Azlan A. A, Rahmah N. and K. A. R: Advance Materials Research. Vol. 364 (2012) p.30.

Google Scholar

[7] Stahlmecke. B., Wagener. S., Asbach. C., Kaminski. H., Fissan. H. and Kuhlbusch. T: J. Nanopart. Res. Vol. 11 (2009), pp.1625-1635.

DOI: 10.1007/s11051-009-9731-x

Google Scholar

[8] Maver, U., Bele, M., Makovec, D., Äoeampelj, S., Jamnik, J. & Gaberå¡ÄEk, M: J. Magn. Magn. Mater. Vol. 321 (2009), pp.3187-3192.

Google Scholar

[9] Chen, R., G. Song, and Y. Wei: J. Phys. Chem. C. Vol. 114 (2010), pp.13409-13413.

Google Scholar