Study on the Fermentation Conditions of β-Glucosidase from Aspergillus niger

Shao Hua Luo; Chao Zhang; Jin Ling Guo; Hua Shun Yu; Juan Yao; Da Chun Gong

doi:10.4028/www.scientific.net/AMR.1033-1034.133

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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 1033-1034Study on the Fermentation Conditions of...

Study on the Fermentation Conditions of β-Glucosidase from Aspergillus niger

Abstract:

Biomass energy is environmentally friendly, renewable, and a rich stock.The research and development of biomass energy have become a hot spot. The β-glucosidase is the key enzyme in the utilization of biomass. The fermentation conditions for β-glucosidase production from Aspergillus niger were optimized. The optimum conditions were as follows: the seed age 48h, the inoculation amount 10%, the medium volume 20mL / 250mL, the initial pH 5.0, the rotational speed 200 r/ min, Under these conditions, the activity of β-glucosidase can research 13.92 U ∕ mL after 168h, increasing 92.8% than before. This experiment can provide the basis for the next step of liquid fermentation for the scaling up production of β-glucosidase.