Determination of Fluoroquinolones Residual in Freshwater Fish by HPLC

Yue Jin; Wen Yu Zhang; Qian Wang; Yi Qing Yang; Li Ya Liang; Shi Jie Yan

doi:10.4028/www.scientific.net/AMR.1033-1034.634

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Determination of Fluoroquinolones Residual in Freshwater Fish by HPLC

Abstract:

HPLC (High Performance Liquid Chromatography) method for danofloxacin(DAN), enrofloxacin(ENR), sarafloxacin(SAR) and difloxacin(DIF) residues in freshwater fish was developed. The fish sample was extracted with methanol-water-acetic acid(2/8/0.01, V/V/V), performed on a C18 solid-phase extraction column. With methanol+malonic acid-magnesium chloride aqueous solution as mobile phase, gradient elution at 30°C, inject volume was 20μL. The wavelength of UV detector was 280 nm. The linear rang of four drugs was 0.1~10μg/mL. In the level of 0.5μg/kg and 4.0μg/kg, the average recovery of the four kinds of drugs was 79.81%~92.03% , the RSD was 1.03%~4.07%. The limit of DAN, ENR, SAR, and DIF was 0.1μg/kg, 0.1μg/kg, 0.05μg/kg, and 0.05μg/kg individual. This method was employed to analyze freshwater fishes, such as catfish, chub and carp, because of its simplicity and high efficiency.