Loading of siRNA on Magnetic PLL-Fe₂O₃@SiO₂ Nanocomposites and their Transfection In Vitro

Abstract:

The magnetic Fe₂O₃ nanoparticles were prepared by co-precipitation method with FeCl₃ and NaOH as starting reagents. The surface of Fe₂O₃ nanoparticles was modified with tetraethyl orthosilicate. Fe₂O₃@SiO₂ nanocomposites were calcined at 600 °C. The nanocomposites were characterized by scanning electron microscope (SEM), transmission electron microscope (TEM), X-ray diffraction (XRD) and energy dispersive X-ray spectroscopy (EDX). The PLL-Fe₂O₃@SiO₂ (SMNP) was prepared by modifying with poly-L-lysine on the surface. The SMNP combined with plasmid siRNA by static electrical charges as one of gene carriers was transfected into SD rat neurons. The results of fluorescence microscope and Prussian blue staining show that SMNP can effectively enter cells. Therefore, SMNP are one kind of novel and effective gene carriers, it can transfect the plasmid which carries the siRNA into SD rats neurons in vitro.

• Periodical: Advanced Materials Research (Volume 1053)
• Pages: 444-449
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.1053.444
• Online since: October 2014
• Authors: Xue Wen Cui, Gang Cheng, Rui Jiang Liu, Li Wei Wang, Yan Shuai Wang
• Keywords: Magnetic Nanocomposite, PLL, PLL-Fe₂O₃@SiO₂, siRNA, Transfection

References

[1] C. Napoli, C. Lemieux and R. Jorgensen, Introduction of a chimeric chalcone synthase gene into petunia results in reversible co-suppression of homologous genes in trans, Plant Cell. 2 (1990) 279-289.

DOI: 10.1105/tpc.2.4.279

[2] A. Fire, S. Xu, M.K. Montgomery, S.A. Kostas, S.E. Driver and C.C. Mello, Potent and specific genetic interference by double-stranded RNA in caenorhabditis elegans, Nature. 391 (1998) 806-811.

DOI: 10.1038/35888

[3] P.D. Zamore, T. Tuschl, P.A. Sharp and D.P. Bartel, RNAi: double-stranded RNA directs the ATP-dependent cleavage of mRNA at 21 to 23 nucleotide intervals, Cell. 101 (2000) 25-33.

DOI: 10.1016/s0092-8674(00)80620-0

[4] C.X. Li, A. Parker, E. Menocal, S. Xiang, L. Borodyansky and J.H. Fruehauf, Delivery of RNA interference, Cell Cycle. 5 (2006) 2103-2109.

DOI: 10.4161/cc.5.18.3192

[5] H. Cohen, R.J. Levy, J. Gao, I. Fishbein, V. Kousaev, S. Sosnowski, S. Slomkowski and G. Golomb, Sustained delivery and expression of DNA encapsulated in polymeric nanoparticles, Gene Ther. 7 (2000) 1896-(1905).

DOI: 10.1038/sj.gt.3301318

[6] J.F. Engelhardt, X. Ye, B. Doranz and J.M. Wilson, Ablation of E2A in recombinant adenoviruses improves transgene persistence and decreases inflammatory response in mouse liver, Proc Natl Acad Sci USA. 91 (1994) 6196-6200.

DOI: 10.1073/pnas.91.13.6196

[7] E.H. Chowdhury. Nuclear targeting of viral and non-viral DNA, Expert Opin Drug Deliv. 6 (2009) 697-703.

DOI: 10.1517/17425240903025744

[8] D.G. Anderson, W.D. Peng, A. Akinc, N. Hossain, A. Kohn, R. Padera, R. Langer and A. Janet, A polymer library approach to suicide gene therapy for cancer, Proc Natl Acad Sci USA. 101 (2004) 16028-16033.

DOI: 10.1073/pnas.0407218101

[9] P. Chollet, M.C. Favrot, A. Hurbin and J.L. Coll, Side-effects of a systemic injection of linear polyethylenimine-DNA complexes, J Gene Med. 4 (2002) 84-91.

DOI: 10.1002/jgm.237

[10] Z. Ma, J. Li, F. He, A. Wilson, B. Pitt and S. Li, Cationic lipids enhance siRNA-mediated interferon response in mice, Biochem Biophys Res Commun. 330 (2005) 755-759.

DOI: 10.1016/j.bbrc.2005.03.041

[11] S. Nie, Y. Xing, G.J. Kim and J.W. Simons, Nanotechnology applications in cancer, Annu Rev Biomed Eng. 9 (2007) 257-288.

DOI: 10.1146/annurev.bioeng.9.060906.152025

[12] M. Mahmoudi, S. Sant, B. Wang, S. Laurent and T. Sen, Superparamagnetic iron oxide nanoparticles (SPIONs): Development, surface modification and applications in chemotherapy, Adv Drug Deliv Rev. 63 (2011) 24-46.

DOI: 10.1016/j.addr.2010.05.006

[13] M.M. Lin, H.H. Kim, H. Kim, J. Dobson and K. do Kim, Surface activation and targeting strategies of superparamagnetic iron oxide nanoparticles in cancer-oriented diagnosis and therapy, Nanomedicine (Lond). 5 (2010) 109-133.

DOI: 10.2217/nnm.09.96

[14] W.L. Gui, Comparative study on preparation of contrast agent for abdomen MRI from superparamagnetic Fe2O3 and Fe3O4 microparticles, Research and Exploration in Laboratory. 27 (2008) 32-33, 95.

[15] Y.J. Yao, S.D. Miao, S.M. Yu, L.P. Ma, H.Q. Sun and S.B. Wang, Fabrication of Fe3O4/SiO2 core/shell nanoparticles attached to graphene oxide and its use as an adsorbent, J Colloid Interface Sci. 379 (2012) 20-26.

DOI: 10.1016/j.jcis.2012.04.030

[16] Z.Y. Lu, J. Dai, X.N. Song, G. Wang and W.S. Yang, Facile synthesis of Fe3O4/SiO2 composite nanoparticles from primary silica particles, A- Physicochem. Eng. Asp. 317 (2008) 450-456.

DOI: 10.1016/j.colsurfa.2007.11.020

[17] Y. Lu, Y.D. Yin, B.T. Mayers and Y.N. Xia, Modifying the surface properties of superparamagnetic iron oxide nanoparticles through a sol-gel approach, Nano Lett. 2 (2002) 183-186.

DOI: 10.1021/nl015681q

[18] C. Hui, C. Shen, J. Tian, L. Bao, H. Ding, C. Li, Y. Tian, X. Shi and H.J. Gao, Core-shell Fe3O4@SiO2 nanoparticles synthesized with well-dispersed hydrophilic Fe3O4 seeds, Nanoscale. 3 (2011) 701-705.

DOI: 10.1039/c0nr00497a