Effect of Carbon Sources and Fe³⁺ on the Growth and Lipid Accumulation of Monoraphidium sp. FXY-10 under Mixotrophic Culture Condition

Abstract:

Effects of different carbon source and Fe³⁺ for the growth and lipid accumulation of Monoraphidium sp. FXY-10 cultured mixotrophically was studied in our present work. The final cell density was reached to 2.626 g L^-1 when glucose was the only carbon source, which is 1.43-fold to sodium acetate (1.834 g L^-1), far higher than sucrose (0.251 g L^-1) and xylitol (0.471 g L^-1), but barely grow in other culture condition. Additionally, the highest algae lipid productivity (77.45 mg L^-1 d^-1) was obtained in 10 g L^-1 glucose group, which indicated that glucose was the optimal carbon source for growth and lipid accumulation of Monoraphidium sp. FXY-10. Nevertheless, Monoraphidium sp. FXY-10 was grew slowly in BG-11 culture medium with the absence of Fe³⁺. The biomass was achieved at the top with 50μM Fe³⁺ added. With the increase of Fe³⁺ concentration, it showed no variation in the growth of microalgae. The highest biomass productivity (209.87 mg L^-1 d^-1) was reached when the Fe³⁺ concentration was at 150μM while highest lipid productivity (94.05 mg L^-1 d^-1) reached at 50μM, which indicated that Fe³⁺ was one of the most indispensable trace elements for the growth and lipid accumulation of Monoraphidium sp. FXY-10.

• Periodical: Advanced Materials Research (Volumes 1070-1072)
• Pages: 157-163
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.1070-1072.157
• Online since: December 2014
• Authors: Hao Miao Jiangwang, Li Huang, Xu Ya Yu*
• Keywords: Carbon Source, Fe³⁺, Growth, Lipid Accumulation, Monoraphidium sp.FXY-10

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