Study of MWNTS Influence upon Liver Histological and Histochemical Parameters in Laboratory Mice: Preliminary Results

Abstract:

Preliminary evaluation of toxic effect of commercially manufactured carbon nanostructured material based on multi-walled carbon nanotubes (MWCNT) upon laboratory mice C57B6/DBA2 males was carried out. It was found that thirty-day oral administration of nanotubes in doses of 0.3 and 3 mg/kg has no effect on liver condition, while administration of 30 mg/kg leads to formation of inflammatory infiltrates together with hepatocyte structure modification. The obtained results are of potential interest for development of industrial safety standards in nanomaterial handling and for development of standards for reproductive toxicity of carbon nanomaterials.

• Periodical: Advanced Materials Research (Volume 1085)
• Pages: 376-383
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.1085.376
• Online since: February 2015
• Authors: Inna A. Vasyukova, Alexander A. Gusev, Alexey Yu. Ubogov, Anna Godymchuk
• Keywords: Biological Safety, Histochemical Methods, Histological Methods, Infiltrates in Liver, Multi-Walled Carbon Nanotube (MWCNT)

References

[1] E. Navarro, A. Baun, R. Behra, N.B. Hartmann, J. Filser, A.J. Miao et al, Environmental behavior and ecotoxicity of engineered nanoparticles to algae, plants, and fungi. Ecotoxicology. 17 (2008) 372-386.

DOI: 10.1007/s10646-008-0214-0

[2] T.S.Y. Chan, F. Nasser, C.H. St-Denis, H.S. Mandal, P. Ghafari, N. Hadjout-Rabi, N.C. Bols, X. Tang, Carbon nanotube compared with carbon black: effects on bacterial survival against grazing by ciliates and antimicrobial treatments, Nanotoxicology. 7 (2013).

DOI: 10.3109/17435390.2011.652205

[3] N. Musee, M. Thwala, N. Nota, The antibacterial effects of engineered nanomaterials: implications for wastewater treatment plants, J. Environ. Monit. 13 (2011) 1164-1183.

DOI: 10.1039/c1em10023h

[4] A.E. Porter, M. Gass, J.S. Bendall, K. Muller, A. Goode, J.N. Skepper, P.A. Midgley, M. Welland, Uptake of noncytotoxic acid-treated single-walled carbon nanotubes into the cytoplasm of human macrophage cells, ACS Nano. 3 (2009) 1485-1492.

DOI: 10.1021/nn900416z

[5] J.K. Lee, B.C. Sayers, K. -S. Chun, H. -C. Lao, J.K. Shipley-Phillips, J.C. Bonner, R. Langenbach, Multi-walled carbon nanotubes induce COX-2 and iNOS expression via MAP Kinase-dependent and -independent mechanisms in mouse RAW264. 7 macrophages, Part. Fiber Toxicol. 9 (2012).

DOI: 10.1186/1743-8977-9-14

[6] P. Jackson, N.R. Jacobsen, A. Baun, R. Birkedal, D. Kühnel, K.A. Jensen, U. Vogel, H. Wallin, Bioaccumulation and ecotoxicity of carbon nanotubes, Chem. Central J. 7 (2013) 1-21.

DOI: 10.1186/1752-153x-7-154

[7] N. Kobayashi, M. Naya, M. Ema, S. Endoh, J. Maru, K. Mizuno, J. Nakanishi, Biological response and morphological assessment of individually dispersed multi-wall carbon nanotubes in the lung after intratracheal instillation in rats, Toxicol. 276 (2010).

DOI: 10.1016/j.tox.2010.07.021

[8] J.G. Teeguarden, B.J. Webb-Robertson, K.M. Waters, A.R. Murray, E.R. Kisin, S.M. Varnum, J.M. Jacobs, J.G. Pounds, R.C. Zanger, A.A. Shvedova, Comparative proteomics and pulmonary toxicity of instilled single-walled carbon nanotubes, crocidolite asbestos, and ultrafine carbon black in mice, Toxicol. Sci. 120 (2011).

DOI: 10.1093/toxsci/kfq363

[9] I. Huizar, A. Malur, J. Patel, M. McPeek, L. Dobbs, C. Wingard, B.P. Barna, M.J. Thomassen, The role of PPARγ in carbon nanotube-elicited granulomatous lung inflammation, Respiratory Research 14 (2013) 1-10.

DOI: 10.1186/1465-9921-14-7

[10] D.B. Warheit, B.R. Laurence, K.L. Reed, D.H. Roach, G.A. Reynolds, T.R. Webb Comparative pulmonary toxicity assessment of single-wall carbon nanotubes in rats, Toxicol. Sci. 77 (2004) 117-125.

DOI: 10.1093/toxsci/kfg228

[11] V.E. Kagan, N.V. Konduru, W. Feng, B.L. Allen, J. Conroy, Y. Volkov, I.I. Vlasova, N.A. Belikova, N. Yanamala, A. Kapralov, Y.Y. Tyurina, J. Shi,. E.R. Kisin, A.R. Murray, J. Franks, D. Stolz, P. Gou, J. Klein-Seetharaman, B. Fadeel, A. Star, A.A. Shvedova, Carbon nanotubes degraded by neutrophil myeloperoxidase induce less pulmonary inflammation, Nature Nanotechnol. 5 (2010).

DOI: 10.1038/nnano.2010.44

[12] F.T. Andón, A.A. Kapralov, N. Yanamala, W. Feng, A. Baygan, B.J. Chambers, K. Hultenby, F. Ye, M.S. Toprak, B.D. Brandner, A. Fornara, J. Klein-Seetharaman, G.P. Kotchey, A. Star, A.A. Shvedova, B. Fadeel, V.E. Kagan, Biodegradation of single-walled carbon nanotubes by eosinophil peroxidase, Small. 9 (2013).

DOI: 10.1002/smll.201202508

[13] Z. Lan, W. -X. Yang Nanoparticles and spermatogenesis: how do nanoparticles affect spermatogenesis and penetrate the blood–testis barrier, Nanomed. 7 (2012) 579-596.

DOI: 10.2217/nnm.12.20

[14] Z. Liu, S. Tabakman, K. Welsher, H. Dai, Carbon nanotubes in biology and medicine: in vitro and in vivo. detection, imaging and drug delivery, Nano Res. 2 (2009) 85-120.

DOI: 10.1007/s12274-009-9009-8

[15] S.Y. Madani, N. Naderi, O. Dissanayake, A. Tan, A.M. Seifalian, A new era of cancer treatment: carbon nanotubes as drug delivery tools, Int. J. Nanomed. 6 (2011) 2963-2979.

DOI: 10.2147/ijn.s16923

[16] B.S. Wong, S.L. Yoong, A. Jagusiak, T. Panczyk, H.K. Ho, W.H. Ang, G. Pastorin, Carbon nanotubes for delivery of small molecule drugs, Adv. Drug. Deliv. Rev. 65 (2013) 1964-(2015).

DOI: 10.1016/j.addr.2013.08.005

[17] A. Patlolla, B. McGinnis, P. Tchounwou, Biochemical and histopathological evaluation of functionalized single-walled carbon nanotubes in Swiss-Webster mice, J. Appl. Toxicol. 31 (2011) 75-83.

DOI: 10.1002/jat.1579

[18] M.L. Schipper, N. Nakayama-Ratchford, C.R. Davis, N.W.S. Kam, P. Chu, Z. Liu, X. Sun, H. Dai, S.S. Gambhir, A pilot toxicology study of single-walled carbon nanotubes in a small sample of mice, Nature Nanotechnol., 3 (2008) 216-221.

DOI: 10.1038/nnano.2008.68

[19] S. Jain, V.S. Thakare, M. Das, C. Godugu, A.K. Jain, R. Mathur, K. Chuttani, A.K. Mishra Toxicity of multiwalled carbon nanotubes with end defects critically depends on their functionalization density, Chem. Res. Toxicol. 24 (2011) 2028-(2039).

DOI: 10.1021/tx2003728

[20] L. Lacerda, H. Ali-Boucetta, M.A. Herrero, G. Pastorin, A. Bianco, M. Prato, K. Kostarelos, Tissue histology and physiology following intravenous administration of differenttypes of functionalized multiwalled carbon nanotubes, Nanomedicine (Lond. ). 3 (2008).

DOI: 10.2217/17435889.3.2.149

[21] I.А. Vasyukova, А.А. Gusev, Т.О. Khaliullin, L.М. Fatkhutdinova, А. Yu. Ubogov, Multi-walled carbon nanotubes and their influence on masculine reproduction activity, Nanotechnol. and Occup. Health (in Russian: Nanotechnologii i ohrana zdorovya). 1 (2014).

[22] A.A. Gusev, I.A. Polyakova, E.B. Gorsheneva, A.V. Emeliyanov, S.V. Shutova, O.N. Zayceva, A.V. Shuklinov, E.A. Snegin, A.G. Tkachev, A.V. Fedorov, T.V. Vasilieva, E.A. Smirnova, E.M. Lazareva, G.E. Onishenko, Reproductive toxicity of carbon nanostructured material - a promising carrier of drugs in laboratory mice, J. Phys.: Conf. Ser. 291 (2011).

DOI: 10.1088/1742-6596/291/1/012052

[23] A.A. Gusev, I.A. Polyakova, E.B. Gorsheneva, A.G. Tkachev, A.V. Emeliyanov, S.V. Shutova, O.N. Zayceva, А A.V. Fedorov, T.V. Vasilieva, Sex differences in physiological effect of carbon nanostructured material as potential carrier of drugs: lab-scale experiments, Nauchniye vedomosti Belgorodskogo gosudarstvennogo universiteta. Seriya Estestvenniye nauki (in Russian). 21 (2010).

DOI: 10.1088/1742-6596/291/1/012052

[24] P.I. Sidorova, Use of laboratory animals in toxicity testing. Guidence, RAMN, Arhangelsk, 2002 (in Russian).

[25] S.B. Fadeev, D.V. Volkov, Integral estimation of animals' state in surgery experiments, Vestnik OGU (in Russian). 1 (2013) 147-150.

[26] Guide for the care and use of laboratory animals, Eighth Edition, The national academies press, Washington, (2011).

[27] H. Hedrich, The Laboratory Mouse, Handbook of Experimental Animals, Academic Press, San Diego, (2012).

[28] I.V. Sanotsky, Methods of toxicity and safety estimation of chemicals, Meditsina, Moskva, 1970 (in Russian).

[29] D.S. Sarkisov, Yu.L. Petrov, Microscopic equipment, Meditsina, Moskva, 1996 (in Russian).

[30] P.F. Rokitskiy, Biological statistics, third ed., Visheishaya shkola, Minsk, 1973 (in Russian).

[31] L.A. Vasilyeva, Statistical methods in biology and farming, NGU Novosiborsk, 2007 (in Russian).

[32] L. Tabet, C. Bussy, N. Amara, A. Setyan, A. Grodet, M. J Rossi., J.C. Pairon, J. Boczkowski, S. Lanone, Adverse effects of industrial multiwalled carbon nanotubes on human pulmonary cells, J. Toxicol. Environ. Health A. 72 (2009) 60-73.

DOI: 10.1080/15287390802476991

[33] A.A. Shvedova, A. Pietroiusti, B. Fadeel, V.E. Kagan, Mechanisms of carbon nanotube-induced toxicity: focus on oxidative stress, Toxicol. Appl. Pharmacol. 261 (2012) 121-133.

DOI: 10.1016/j.taap.2012.03.023