Analysis of Polychlorinated Biphenyls in Biological Samples by Headspace Solid Phase Microextraction Coupled with Gas Chromatography

Abstract:

Based on headspace solid phase microextraction (HS-SPME) procedure coupled with gas chromatography, a method for determination of polychlorinated biphenyls (PCBs) in biological samples was developed. The optimization of factors influencing the performances of SPME was studied in detail. To perform the HS-SPME polydimethylsiloxane (PDMS), polydimethylsiloxane-divinyl benzene (PDMS-DVB) and polyacrylate (PA) fibers were compared on the basis of their absorption capacities for the selected compounds, and PDMS-DVB fiber was selected. The influence of various parameters on PCBs extraction efficiency by HS-SPME was studied using GC-electron capture detector (ECD). The linear range of most compounds was found to be between 1 and 100 μgL⁻¹ and the limits of detection were between 20.1 and 42.3 ngL⁻¹. The reproducibility of the method (n = 6), expressed as relative standard deviation (RSD), was between 4.63 and 9.71%. Finally, the optimization of method was applied to determine selected PCBs in biological samples.

• Periodical: Advanced Materials Research (Volumes 1092-1093)
• Pages: 770-773
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.1092-1093.770
• Online since: March 2015
• Authors: Yong Li Liu, Xian Yin Ping*
• Keywords: Gas Chromatography, Polychlorinated Biphenyls, Solid Phase Microextraction

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